Renilla Luciferase Recombinant Rabbit mAb
Product Name :
Renilla Luciferase Recombinant Rabbit mAb
Cat.No.:
db11041
Synonyms :
Renilla-luciferin 2-monooxygenase; Renilla-type luciferase
Application :
WB, IHC, ICC/IF, FC, IP
Reactivity :
Other
Host species :
Rabbit
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Background
Upon binding the substrate, the enzyme catalyzes an oxygenation, producing a very short-lived hydroperoxide that cyclizes into a dioxetanone structure, which collapses, releasing a CO2 molecule. The spontaneous breakdown of the dioxetanone releases the energy (about 50 kcal/mole) that is necessary to generate the excited state of the coelenteramide product, which is the singlet form of the monoanion. In vivo the product undergoes the process of nonradiative energy transfer to an accessory protein, a green fluorescent protein (GFP), which results in green bioluminescence. In vitro, in the absence of GFP, the product emits blue light.
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Immunogen
A synthetic peptide of Renilla Luciferase
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Swiss Prot
P27652
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Synonyms
Renilla-luciferin 2-monooxygenase; Renilla-type luciferase
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Reactivity
Other
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Application
WB, IHC, ICC/IF, FC, IP
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Recommended dilution
WB: 1:2000
ICC/IF: 1:200
FC: 1:50
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Calculated MW
36 kDa
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Observed MW
36 kDa
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Host species
Rabbit
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Clonality
Monoclonal
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Isotype
IgG
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Purity
Affinity Purification
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Conjugation
Un-conjugated
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Storage/Stability
Store at -20°C. Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40%Glycerol, 0.01% sodium azide and 0.05% BSA. Stable for 12 months from date of receipt.
订购信息
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Package
Price (RMB)
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100μL
1200
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500μL
4800
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1mL
8600
- 货期:现货
For research use only. Not intended for diagnostic.