Renilla Luciferase Rabbit pAb
Product Name :
Renilla Luciferase Rabbit pAb
Cat.No.:
db3911
Synonyms :
Renilla Luciferase
Application :
WB, IHC, ICC/IF, FC
Reactivity :
Other
Host species :
Rabbit
-
Background
Upon binding the substrate, the enzyme catalyzes an oxygenation, producing a very short-lived hydroperoxide that cyclizes into a dioxetanone structure, which collapses, releasing a CO2 molecule. The spontaneous breakdown of the dioxetanone releases the energy (about 50 kcal/mole) that is necessary to generate the excited state of the coelenteramide product, which is the singlet form of the monoanion. In vivo the product undergoes the process of nonradiative energy transfer to an accessory protein, a green fluorescent protein (GFP), which results in green bioluminescence. In vitro, in the absence of GFP, the product emits blue light.
-
Immunogen
A synthetic peptide of Renilla Luciferase
-
Swiss Prot
P27652
-
Synonyms
Renilla Luciferase
-
Reactivity
Other
-
Application
WB, IHC, ICC/IF, FC
-
Recommended dilution
WB: 1:1000
ICC/IF: 1:200
-
Calculated MW
36 kDa
-
Observed MW
According to the fused protein
-
Host species
Rabbit
-
Clonality
Polyclonal
-
Isotype
IgG
-
Purity
Affinity Purification
-
Conjugation
Un-conjugated
-
Storage/Stability
Store at -20°C. Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40%Glycerol, 0.01% sodium azide and 0.05% BSA. Stable for 12 months from date of receipt.
订购信息
-
Package
Price (RMB)
-
100μL
1000
-
500μL
4000
-
1mL
7200
- 货期:现货
For research use only. Not intended for diagnostic.