Renilla Luciferase Rabbit pAb - diagbio | 戴格生物

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Renilla Luciferase Rabbit pAb

db67

Product Name : Renilla Luciferase Rabbit pAb

Cat.No.: db67

Synonyms : Renilla-luciferin 2-monooxygenase; Renilla-type luciferase

Application : WB, IHC, ICC/IF, FC, IP

Reactivity : Other

Host species : Rabbit

Background Upon binding the substrate, the enzyme catalyzes an oxygenation, producing a very short-lived hydroperoxide that cyclizes into a dioxetanone structure, which collapses, releasing a CO2 molecule. The spontaneous breakdown of the dioxetanone releases the energy (about 50 kcal/mole) that is necessary to generate the excited state of the coelenteramide product, which is the singlet form of the monoanion. In vivo the product undergoes the process of nonradiative energy transfer to an accessory protein, a green fluorescent protein (GFP), which results in green bioluminescence. In vitro, in the absence of GFP, the product emits blue light.
Immunogen A synthetic peptide of Renilla Luciferase
Swiss Prot P27652
Synonyms Renilla-luciferin 2-monooxygenase; Renilla-type luciferase
Reactivity Other
Application WB, IHC, ICC/IF, FC, IP
Recommended dilution WB: 1:2000
ICC/IF: 1:200
FC: 1:50
Calculated MW 36 kDa
Observed MW 36 kDa
Host species Rabbit
Clonality Polyclonal
Isotype IgG
Purity Affinity Purification
Conjugation Un-conjugated
Storage/Stability Store at -20°C. Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40%Glycerol, 0.01% sodium azide and 0.05% BSA. Stable for 12 months from date of receipt.

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