您当前的位置：
alpha B Crystallin (DGR12950) Rabbit mAb
db11173
Datasheet
- Background Mammalian lens crystallins are divided into alpha, beta, and gamma families. Alpha crystallins are composed of two gene products: alpha-A and alpha-B, for acidic and basic, respectively. Alpha crystallins can be induced by heat shock and are members of the small heat shock protein (HSP20) family. They act as molecular chaperones although they do not renature proteins and release them in the fashion of a true chaperone; instead they hold them in large soluble aggregates. Post-translational modifications decrease the ability to chaperone. These heterogeneous aggregates consist of 30-40 subunits; the alpha-A and alpha-B subunits have a 3:1 ratio, respectively. Two additional functions of alpha crystallins are an autokinase activity and participation in the intracellular architecture. The encoded protein has been identified as a moonlighting protein based on its ability to perform mechanistically distinct functions. Alpha-A and alpha-B gene products are differentially expressed; alpha-A is preferentially restricted to the lens and alpha-B is expressed widely in many tissues and organs. Elevated expression of alpha-B crystallin occurs in many neurological diseases; a missense mutation cosegregated in a family with a desmin-related myopathy. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jan 2014]
- Immunogen A synthetic peptide of human Alpha B Crystallin
-
Gene ID
1410
-
Swiss Prot
P02511
- Synonyms MFM2; CRYA2; CTPP2; HSPB5; CMD1II; CTRCT16; HEL-S-101
- Reactivity Human,Rat
- Application WB, IHC-P, ICC/IF, IP
-
Recommended dilution
WB: 1:1000
IHC-P: 1:100-1:200
ICC/IF: 1:100-1:200
IP: 1:100-1:200 - Calculated MW 20 kDa
- Observed MW 22 kDa
- Host species Rabbit
- Clonality Monoclonal
- Clonality No. DGR12950
- Isotype IgG
- Purity Affinity Purification
- Conjugation Un-conjugated
- Storage/Stability Store at -20°C. Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA. Stable for 12 months from date of receipt.
-
Western blot detection of Alpha B Crystallin in C6,Hela cell lysates using Alpha B Crystallin antibody(1:1000 diluted). -
Immunofluorescent analysis of HeLa cells using db11173 antibody (green), and DAPI (blue).
订购信息
- Package Price (RMB)
- 20μL 698
- 50μL 1398
- 100μL 2398
- 货期：现货
相关产品
For research use only. Not intended for diagnostic.
