产品中心 Products Center
Phospho-AKT1 (Ser473) Recombinant Rabbit mAb
Alternate Text
db14851
Datasheet

Product Name : Phospho-AKT1 (Ser473) Recombinant Rabbit mAb

Cat.No.: db14851

Synonyms : AKT; PKB; RAC; CWS6; PRKBA; PKB-ALPHA; RAC-ALPHA

Application : WB, IHC, ICC/IF

Reactivity : Human, Mouse, Rat

Host species : Rabbit

  • Background The serine-threonine protein kinase encoded by the AKT1 gene is catalytically inactive in serum-starved primary and immortalized fibroblasts. AKT1 and the related AKT2 are activated by platelet-derived growth factor. The activation is rapid and specific, and it is abrogated by mutations in the pleckstrin homology domain of AKT1. It was shown that the activation occurs through phosphatidylinositol 3-kinase. In the developing nervous system AKT is a critical mediator of growth factor-induced neuronal survival. Survival factors can suppress apoptosis in a transcription-independent manner by activating the serine/threonine kinase AKT1, which then phosphorylates and inactivates components of the apoptotic machinery. Mutations in this gene have been associated with the Proteus syndrome. Multiple alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Jul 2011]
  • Immunogen A synthetic phosphopeptide corresponding to residues surrounding Ser473 of human AKT1
  • Gene ID 207
  • Swiss Prot P31749
  • Synonyms AKT; PKB; RAC; CWS6; PRKBA; PKB-ALPHA; RAC-ALPHA
  • Reactivity Human, Mouse, Rat
  • Application WB, IHC, ICC/IF
  • Recommended dilution WB: 1:1000
    IHC: 1:50
    ICC/IF: 1:50
  • Calculated MW 56 kDa
  • Observed MW 56 kDa
  • Host species Rabbit
  • Clonality Monoclonal
  • Isotype IgG
  • Purity Affinity Purification
  • Conjugation Un-conjugated
  • Storage/Stability Store at -20°C. Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40%Glycerol, 0.01% sodium azide and 0.05% BSA. Stable for 12 months from date of receipt.
  • Western blot detection of Phospho-AKT (Ser473) in C6,3T3,Hela cell lysates using Phospho-AKT (Ser473) antibody(1:1000 diluted).
订购信息
  • Package Price (RMB)
  • 20μL 698
  • 50μL 1398
  • 100μL 2398
  • 货期:现货
For research use only. Not intended for diagnostic.