Product Name :
BRG1 Rabbit pAb
Cat.No.:
db3420
Synonyms :
Brg1; SW1/SNF; HP1-BP72; SNF2beta; b2b692Clo; b2b508.1Clo
Application :
WB, IHC, ICC/IF, FC, IP, ChIP
Reactivity :
Human, Mouse, Rat
Host species :
Rabbit
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Background
Transcriptional coactivator cooperating with nuclear hormone receptors to potentiate transcriptional activation. Component of the CREST-BRG1 complex, a multiprotein complex that regulates promoter activation by orchestrating a calcium-dependent release of a repressor complex and a recruitment of an activator complex. In resting neurons, transcription of the c-FOS promoter is inhibited by BRG1-dependent recruitment of a phospho-RB1-HDAC repressor complex. Upon calcium influx, RB1 is dephosphorylated by calcineurin, which leads to release of the repressor complex. At the same time, there is increased recruitment of CREBBP to the promoter by a CREST-dependent mechanism, which leads to transcriptional activation. The CREST-BRG1 complex also binds to the NR2B promoter, and activity-dependent induction of NR2B expression involves a release of HDAC1 and recruitment of CREBBP (By similarity).
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Immunogen
A synthetic peptide of mouse BRG1
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Gene ID
20586
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Swiss Prot
Q3TKT4
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Synonyms
Brg1; SW1/SNF; HP1-BP72; SNF2beta; b2b692Clo; b2b508.1Clo
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Reactivity
Human, Mouse, Rat
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Application
WB, IHC, ICC/IF, FC, IP, ChIP
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Recommended dilution
WB: 1:2000-1:10000
IHC: 1:20-1:100
ICC/IF: 1:50
FC: 1:20
IP: 1:20-1:50
ChIP: 1:20
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Calculated MW
185 kDa
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Observed MW
220 kDa
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Host species
Rabbit
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Clonality
Polyclonal
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Isotype
IgG
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Purity
Affinity Purification
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Conjugation
Un-conjugated
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Storage/Stability
Store at -20°C. Supplied in 50nM Tris-Glycine(pH 7.4), 0.15M NaCl, 40%Glycerol, 0.01% sodium azide and 0.05% BSA. Stable for 12 months from date of receipt.
订购信息
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Package
Price (RMB)
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20μL
598
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50μL
998
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100μL
1798
- 货期：现货
For research use only. Not intended for diagnostic.
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