PHD3 Rabbit pAb - diagbio | 戴格生物

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PHD3 Rabbit pAb

db5389

Product Name : PHD3 Rabbit pAb

Cat.No.: db5389

Synonyms : PHD3; HIFPH3; HIFP4H3

Application : WB, IHC, ICC/IF, IP

Reactivity : Human, Mouse, Rat

Host species : Rabbit

Background Cellular oxygen sensor that catalyzes, under normoxic conditions, the post-translational formation of 4-hydroxyproline in hypoxia-inducible factor (HIF) alpha proteins. Hydroxylates a specific proline found in each of the oxygen-dependent degradation (ODD) domains (N-terminal, NODD, and C-terminal, CODD) of HIF1A. Also hydroxylates HIF2A. Has a preference for the CODD site for both HIF1A and HIF2A. Hydroxylation on the NODD site by EGLN3 appears to require prior hydroxylation on the CODD site. Hydroxylated HIFs are then targeted for proteasomal degradation via the von Hippel-Lindau ubiquitination complex. Under hypoxic conditions, the hydroxylation reaction is attenuated allowing HIFs to escape degradation resulting in their translocation to the nucleus, heterodimerization with HIF1B, and increased expression of hypoxy-inducible genes.
Immunogen Recombinant protein of human PHD3
Gene ID 112399
Swiss Prot Q9H6Z9
Synonyms PHD3; HIFPH3; HIFP4H3
Reactivity Human, Mouse, Rat
Application WB, IHC, ICC/IF, IP
Recommended dilution WB: 1:1000
IHC: 1:50
ICC/IF: 1:50
IP: 1:20
Calculated MW 27 kDa
Observed MW 27 kDa
Host species Rabbit
Clonality Polyclonal
Isotype IgG
Purity Affinity Purification
Conjugation Un-conjugated
Storage/Stability Store at -20°C. Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40%Glycerol, 0.01% sodium azide and 0.05% BSA. Stable for 12 months from date of receipt.

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