Phospho-Histone H2A.X (Ser139) (2A9) Mouse mAb - diagbio | 戴格生物

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Phospho-Histone H2A.X (Ser139) (2A9) Mouse mAb

db6249

Product Name : Phospho-Histone H2A.X (Ser139) (2A9) Mouse mAb

Cat.No.: db6249

Synonyms : H2AX; H2AFX; H2a/x; HIST5-2AX; Histone H2AX

Application : WB, ICC/IF

Reactivity : Human, Mouse

Host species : Mouse

Background Variant histone H2A which replaces conventional H2A in a subset of nucleosomes. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. Required for checkpoint-mediated arrest of cell cycle progression in response to low doses of ionizing radiation and for efficient repair of DNA double strand breaks (DSBs) specifically when modified by C-terminal phosphorylation.
Immunogen Synthetic phosphopeptide corresponding to residues surrounding Ser139 of human H2A.X
Gene ID 3014
Swiss Prot P16104
Synonyms H2AX; H2AFX; H2a/x; HIST5-2AX; Histone H2AX
Reactivity Human, Mouse
Application WB, ICC/IF
Recommended dilution WB: 1:500-1:1000
ICC/IF: 1:50-1:200
Calculated MW 15 kDa
Observed MW 15 kDa
Host species Mouse
Clonality Monoclonal
Clonality No. 2A9-B5
Isotype IgG2a
Purity Affinity Purification
Conjugation Un-conjugated
Storage/Stability Store at -20°C. Supplied in PBS, 50% glycerol, 0.5% BSA and 0.02% sodium azide, pH 7.3. Stable for 12 months from date of receipt.

Western blot analysis of Phosphorylation of H2A.X at Serine 139 in 3T3 or Hydroxyureatreated 3T3 lysates using Phospho-Histone H2A.X (Ser139) antibody.
Immunofluorescence analysis of Phospho-Histone H2A.X (Ser139) (2A9) in 3T3 or Hydroxyureatreated 3T3 using Phospho-Histone H2A.X (Ser139) (2A9) antibody
Immunofluorescence analysis of Phospho-Histone H2A.X (Ser139) (2A9) in A549(upper, untreated or Hydroxyureatreated) and Hela(lower, untreated or Hydroxyureatreated) using Phospho-Histone H2A.X (Ser139) antibody.
Western blot analysis of Phospho-Histone H2A.X (Ser139) (2A9) in untreated or Hydroxyureatreated Hela and A549 lysates using Phospho-Histone H2A.X (Ser139) (2A9) antibody(upper) or betaActin antibody(2000688F10) (lower).

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