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NF-kB p65 (3D2) Mouse mAb

Product Name : NF-kB p65 (3D2) Mouse mAb

Cat.No.: db6456

Synonyms : NFKB3; RELA; TF65; Transcription factor p65; p65; NFkB

Application : WB, IHC-F, IHC-P, ICC/IF, IP

Reactivity : Human, Mouse, Rat

Host species : Mouse

  • Background NF-kappa-B is a pleiotropic transcription factor present in almost all cell types and is the endpoint of a series of signal transduction events that are initiated by a vast array of stimuli related to many biological processes such as inflammation, immunity, differentiation, cell growth, tumorigenesis and apoptosis. NF-kappa-B is a homo- or heterodimeric complex formed by the Rel-like domain-containing proteins RELA/p65, RELB, NFKB1/p105, NFKB1/p50, REL and NFKB2/p52 and the heterodimeric p65-p50 complex appears to be most abundant one. The dimers bind at kappa-B sites in the DNA of their target genes and the individual dimers have distinct preferences for different kappa-B sites that they can bind with distinguishable affinity and specificity. Different dimer combinations act as transcriptional activators or repressors, respectively. NF-kappa-B is controlled by various mechanisms of post-translational modification and subcellular compartmentalization as well as by interactions with other cofactors or corepressors. NF-kappa-B complexes are held in the cytoplasm in an inactive state complexed with members of the NF-kappa-B inhibitor (I-kappa-B) family. In a conventional activation pathway, I-kappa-B is phosphorylated by I-kappa-B kinases (IKKs) in response to different activators, subsequently degraded thus liberating the active NF-kappa-B complex which translocates to the nucleus. NF-kappa-B heterodimeric p65-p50 and p65-c-Rel complexes are transcriptional activators. The NF-kappa-B p65-p65 complex appears to be involved in invasin-mediated activation of IL-8 expression. The inhibitory effect of I-kappa-B upon NF-kappa-B the cytoplasm is exerted primarily through the interaction with p65. p65 shows a weak DNA-binding site which could contribute directly to DNA binding in the NF-kappa-B complex. Associates with chromatin at the NF-kappa-B promoter region via association with DDX1. Essential for cytokine gene expression in T-cells (PubMed/15790681).
  • Immunogen Recombinant Protein of Transcription factor p65
  • Gene ID 5970
  • Swiss Prot Q04206
  • Synonyms NFKB3; RELA; TF65; Transcription factor p65; p65; NFkB
  • Reactivity Human, Mouse, Rat
  • Application WB, IHC-F, IHC-P, ICC/IF, IP
  • Recommended dilution WB: 1:500-1000
    IP: 1:20
    ICC/IF: 1:50-1:200
    IHC: 1:50-100
  • Calculated MW 60 kDa
  • Observed MW 65 kDa
  • Host species Mouse
  • Clonality Monoclonal
  • Clonality No. 3D2-4E9-7A8
  • Isotype IgG1
  • Purity Affinity Purification
  • Conjugation Un-conjugated
  • Storage/Stability Store at -20°C. Supplied in PBS, 50% glycerol, 0.5% BSA and 0.02% sodium azide, pH 7.3. Stable for 12 months from date of receipt.
  • Western blot analysis of NF-KB p65 in T47D, 3T3, COS7, C6 and Hela lysates using NF-KB p65 antibody.
  • Immunofluorescence analysis of NF-KB p65 (3D2) in Hela using NF-KB p65 (3D2) antibody,and DAPI (blue).
  • Immunoprecipitation analysis of NF-KB p65 (3D2) in Hela lysates using NFκB p65 (3D2) antibody.
  • Immunohistochemistry analysis of paraffin-embedded rat Brain Tissue using NF-KB p65 antibody.High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.
  • Western blot analysis of NF-KB p65 (3D2) in COS7, C6, T47D lysates using NF-KB p65 (3D2) antibody.
订购信息
  • Package Price (RMB)
  • 50μL 1398
  • 100μL 2398
  • 货期:现货
For research use only. Not intended for diagnostic.