Cytochrome C (7C10) Mouse mAb - diagbio | 戴格生物

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Cytochrome C (7C10) Mouse mAb

db6489

Product Name : Cytochrome C (7C10) Mouse mAb

Cat.No.: db6489

Synonyms : CYCS; CYC; Cytochrome c

Application : WB, ICC/IF, IHC-P

Reactivity : Human, Mouse, Rat, Chicken

Host species : Mouse

Background Electron carrier protein. The oxidized form of the cytochrome c heme group can accept an electron from the heme group of the cytochrome c1 subunit of cytochrome reductase. Cytochrome c then transfers this electron to the cytochrome oxidase complex, the final protein carrier in the mitochondrial electron-transport chain.Plays a role in apoptosis. Suppression of the anti-apoptotic members or activation of the pro-apoptotic members of the Bcl-2 family leads to altered mitochondrial membrane permeability resulting in release of cytochrome c into the cytosol. Binding of cytochrome c to Apaf-1 triggers the activation of caspase-9, which then accelerates apoptosis by activating other caspases.SitesFeature keyPosition(s)Description
Immunogen Recombinant Protein of CYCS
Gene ID 54205
Swiss Prot P99999
Synonyms CYCS; CYC; Cytochrome c
Reactivity Human, Mouse, Rat, Chicken
Application WB, ICC/IF, IHC-P
Recommended dilution WB: 1:500-1000
IHC: 1:50-100
ICC/IF: 1:50-1:200
Calculated MW 12 kDa
Observed MW 12 kDa
Host species Mouse
Clonality Monoclonal
Clonality No. 7C10-3H3-9A3
Isotype IgG1
Purity Affinity Purification
Conjugation Un-conjugated
Storage/Stability Store at -20°C. Supplied in PBS, 50% glycerol, 0.5% BSA and 0.02% sodium azide, pH 7.3. Stable for 12 months from date of receipt.

Western blot analysis of Cytochrome c in T47D, 3T3, COS7, C6 and Hela lysates using Cytochrome c antibody.
Western blot analysis of Cytochrome C (7C10) in Hela, T47D, mouse brain, K562, rat brain lysates using Cytochrome C (7C10) antibody.
Immunocytochemistry analysis of Cytochrome C (7C1) (green) in Hela using Cytochrome C (7C1) antibody ,and DAPI(blue)
Immunohistochemistry analysis of paraffin-embedded uman tonsil tissue using Cytochrome C (7C10) antibody.High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.

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