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Renilla Luciferase (DGR15022) Rabbit mAb
db11042
Datasheet
- Background Upon binding the substrate, the enzyme catalyzes an oxygenation, producing a very short-lived hydroperoxide that cyclizes into a dioxetanone structure, which collapses, releasing a CO2 molecule. The spontaneous breakdown of the dioxetanone releases the energy (about 50 kcal/mole) that is necessary to generate the excited state of the coelenteramide product, which is the singlet form of the monoanion. In vivo the product undergoes the process of nonradiative energy transfer to an accessory protein, a green fluorescent protein (GFP), which results in green bioluminescence. In vitro, in the absence of GFP, the product emits blue light.
- Immunogen A synthetic peptide of Renilla Luciferase
-
Swiss Prot
P27652
- Synonyms Renilla Luciferase
- Reactivity Renilla reniformis
- Application WB, ICC/IF
-
Recommended dilution
WB: 1:1000
ICC/IF: 1:500-1:1000 - Calculated MW 36 kDa
- Observed MW According to the fused protein
- Host species Rabbit
- Clonality Monoclonal
- Clonality No. DGR15022
- Isotype IgG
- Purity Affinity Purification
- Conjugation Un-conjugated
- Storage/Stability Store at -20°C. Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA. Stable for 12 months from date of receipt.
订购信息
- Package Price (RMB)
- 10μL 398
- 20μL 698
- 100μL 1200
- 500μL 4800
- 1mL 8600
- 货期：现货
相关产品
For research use only. Not intended for diagnostic.
