VSV G Tag (4A10) Mouse mAb - diagbio | 戴格生物

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VSV G Tag (4A10) Mouse mAb

db6411

Datasheet

Product Name : VSV G Tag (4A10) Mouse mAb

Cat.No.: db6411

Synonyms : VSV tag; VSVG tag

Application : WB, IP, ICC/IF

Reactivity : Transfected

Host species : Mouse

Background The fusiogenic envelope G glycoprotein of the vesicular stomatitis virus (VSV-G) that has been used to pseudotype retrovirus and lentivirus vectors can be used alone as an efficient vehicle for gene transfer. The VSV-G epitope tag is commonly engineered onto the N- or C- terminus of a protein of interest so that the tagged protein can be analyzed and visualized using immunochemical methods.
Immunogen Synthetic Peptide of VSV-G-Tag
Synonyms VSV tag; VSVG tag
Reactivity Transfected
Application WB, IP, ICC/IF
Recommended dilution WB: 1:1000-1:10000
ICC/IF: 1:100-1:500
IP: 1:20
Observed MW According to the fused protein
Host species Mouse
Clonality Monoclonal
Clonality No. 4A10-9A6-6H5
Isotype IgG1
Purity Affinity Purification
Conjugation Un-conjugated
Storage/Stability Store at -20°C. Supplied in PBS, 50% Glycerol(pH 7.3), 0.02% sodium azide and 0.5% BSA . Stable for 12 months from date of receipt.

Western blot analysis of VSV G tag using VSV G tag antibody.
Immunofluorescence analysis of VSV G tag (4A10) in 293T cells transfected with a VSVGtagged protein using VSV G tag (4A10) antibody(red),and DAPI (blue).
IP antibody use: 5ug VSV-G mouse IgG1 per ml Lysate. 1、untransfected 293 cell lysate 2、transfected 293 cell lysate with VSV-G-tag fusion protein 3、IP(untransfted 293+anti-VSV-G mAb+Protein G agarose) 4、IP (transfected 293+ normal mouse IgG+Protein G agarose) 5、IP (transfected 293+anti-VSV-G mAb+ Protein G agarose)

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