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Goat Anti Rabbit IgG (H+L)-AF488
db10005
Datasheet
- Background Secondary antibodies providesignal detection and amplification along with extending the utility of anantibody through conjugation to proteins. Secondary antibodies bind to primaryantibodies, which are directly bound to the target antigen(s).
- Immunogen Rabbit IgG
- Reactivity Rabbit
- Application ICC/IF, IHC-F, IHC-P, FC, ELISA
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Recommended dilution
ICC/IF: 1:200-1:1000
IHC-P: 1:200-1:1000
IHC-F: 1:200-1:1000
FC: 1:200-1:1000
ELISA: 1:2000-1:20000 - Host species Goat
- Clonality Polyclonal
- Isotype IgG
- Purity Affinity Purification
- Conjugation AF488
- Storage/Stability Store at -20°C. Supplied in PBS, 50% Glycerol, 0.1% BSA. Stable for 12 months from date of receipt.
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Immunofluorescence analysis of HeLa cells labelling Met (c-Met) with db15811.
The cells were fixed with 4% PFA (10min, RT) followed by treatment with 0.1% Triton X-100 (10min, RT), and blocked in 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween 20 for 1h. The cells were then incubate with db15811 (1:1000) at room temprature for 1h, followed by a further incubation at room temperature for 45min with Goat Anti Rabbit lgG (H+L)-AF488 (db10005, shown in green) at 1:400 dilution. Nuclear DNA was labeled in blue with DAPI.
Control: Secondary antibody only. -
Immunofluorescence analysis of HeLa cells labelling PODXL with db15764.
The cells were fixed with 4% PFA (10min, RT) followed by treatment with 0.1% Triton X-100 (10min, RT), and blocked in 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween 20 for 1h. The cells were then incubate with db15764 (1:100) at room temprature for 1h, followed by a further incubation at room temperature for 45min with Goat Anti Rabbit lgG (H+L)-AF488 (db10005, shown in green) at 1:400 dilution. Nuclear DNA was labeled in blue with DAPI.
Control: Secondary antibody only. -
Immunofluorescence analysis of HeLa cells labelling Sumo 1 with db15878.
The cells were fixed with 4% PFA (10min, RT) followed by treatment with 0.1% Triton X-100 (10min, RT), and blocked in 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween 20 for 1h. The cells were then incubate with db15878 (1:200) at room temprature for 1h, followed by a further incubation at room temperature for 45min with Goat Anti Rabbit lgG (H+L)-AF488 (db10005, shown in green) at 1:400 dilution. Nuclear DNA was labeled in blue with DAPI.
Control: Secondary antibody only. -
Immunofluorescence analysis of NIH/3T3 cells labelling C7 with db16016.
The cells were fixed with 4% PFA (10min, RT) followed by treatment with 0.1% Triton X-100 (10min, RT), and blocked in 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween 20 for 1h. The cells were then incubate with db16016 (1:200) at room temprature for 1h, followed by a further incubation at room temperature for 45min with Goat Anti Rabbit lgG (H+L)-AF488 (db10005, shown in green) at 1:400 dilution. Nuclear DNA was labeled in blue with DAPI.
Control: Secondary antibody only.
订购信息
- Package Price (RMB)
- 100μL 280
- 货期：现货
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For research use only. Not intended for diagnostic.
