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Conjugation/Formulation：Un-conjugated
- Background This gene encodes a member of the heat shock protein 90 family; these proteins are involved in signal transduction, protein folding and degradation and morphological evolution. This gene encodes the constitutive form of the cytosolic 90 kDa heat-shock protein and is thought to play a role in gastric apoptosis and inflammation. Alternative splicing results in multiple transcript variants. Pseudogenes have been identified on multiple chromosomes. [provided by RefSeq, Dec 2012]
- Immunogen Recombinant protein of human Hsp90 beta
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Gene ID
3326
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Swiss Prot
P08238
- Synonyms HSP84; HSPC2; HSPCB; D6S182; HSP90B
- Reactivity Human,Mouse,Rat
- Application WB, IHC-P, ICC/IF, FC, IP
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Recommended dilution
WB: 1:1000-1:5000
IHC-P: 1:200-1:1000
ICC/IF: 1:100-1:200
FC: 1:200-1:500
IP: 1:100 - Calculated MW 85,83 kDa
- Observed MW 90 kDa
- Host species Rabbit
- Clonality Monoclonal
- Clonality No. DGR12120
- Isotype IgG
- Purity Affinity Purification
- Conjugation Un-conjugated
- Storage/Stability Store at -20°C. Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA. Stable for 12 months from date of receipt.
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Western blot analysis of extracts from C6, 3T3, HeLa cells and Rat brain tissue using db11017 at 1:1000. -
Western blot analysis of extracts from U-2 OS cells and Rat lung tissue using db11017 at 1:6000. -
Immunohistochemical analysis of paraffin-embedded human tonsil using db11017 antibody. -
Immunofluorescence analysis of HeLa cells labelling Hsp90 with db11017.
The cells were fixed with cold 100% methanol (10min, 4℃) and blocked in 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween 20 for 1h. The cells were then incubate with db11017 (1:100) at room temprature for 1h, followed by a further incubation at room temperature for 45min with Goat Anti Rabbit lgG (H+L)-AF488 (db10005, shown in green). Nuclear DNA was labeled in blue with DAPI.
Control: Secondary antibody only.
订购信息
- Package Price (RMB)
- 10μL 398
- 20μL 698
- 50μL 1398
- 100μL 2398
- 货期：现货
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For research use only. Not intended for diagnostic.
