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Histone H3 Recombinant Rabbit mAb
db11049
Datasheet
- Background Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. This structure consists of approximately 146 bp of DNA wrapped around a nucleosome, an octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is found in the large histone gene cluster on chromosome 6p22-p21.3. [provided by RefSeq, Aug 2015]
- Immunogen A synthetic peptide of human Histone H3
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Gene ID
8350
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Swiss Prot
P68431
- Synonyms H3/A; H3FA
- Reactivity Human, Mouse, Rat
- Application WB, IHC, ICC/IF, FC
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Recommended dilution
WB: 1:1000
IHC-P: 1:2000-1:10000
ICC/IF: 1:500-1:2000
FC: 1:50 - Calculated MW 15 kDa
- Observed MW 17 kDa
- Host species Rabbit
- Clonality Monoclonal
- Clonality No. DGR11883
- Isotype IgG
- Purity Affinity Purification
- Conjugation Un-conjugated
- Storage/Stability Store at -20°C. Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA. Stable for 12 months from date of receipt.
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Western blot analysis of extracts from Hela，3T3 cells using db11049 at 1:1000. -
Immunofluorescence analysis of A431 cells labelling Histone H3 with db11049.
The cells were fixed with cold 100% methanol (10min, 4℃) and blocked in 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween 20 for 1h. The cells were then incubate with db11049 (1:500) at room temprature for 1h, followed by a further incubation at room temperature for 45min with Goat Anti Rabbit lgG (H+L)-AF488 (db10005, shown in green). Nuclear DNA was labeled in blue with DAPI.
Control: Secondary antibody only. -
Western blot analysis of extracts from Mouse teatis tissue using db11049 at 1:50000.
订购信息
- Package Price (RMB)
- 10μL 398
- 20μL 698
- 100μL 1200
- 500μL 4800
- 1mL 8600
- 货期：现货
相关产品
For research use only. Not intended for diagnostic.
