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SMARCD1 (DGR34019) Rabbit mAb

Product Name : SMARCD1 (DGR34019) Rabbit mAb

Cat.No.: db11072

Synonyms : CSS11; Rsc6p; BAF60A; CRACD1

Application:

Reactivity : Human, Mouse

Host species : Rabbit

Conjugation/Formulation:Un-conjugated

  • Background The protein encoded by this gene is a member of the SWI/SNF family of proteins, whose members display helicase and ATPase activities and which are thought to regulate transcription of certain genes by altering the chromatin structure around those genes. The encoded protein is part of the large ATP-dependent chromatin remodeling complex SNF/SWI and has sequence similarity to the yeast Swp73 protein. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
  • Immunogen A synthetic peptide of human SMARCD1
  • Gene ID 6602
  • Swiss Prot Q96GM5
  • Synonyms CSS11; Rsc6p; BAF60A; CRACD1
  • Reactivity Human,Mouse
  • Application WB, ICC/IF, FC, IP
  • Recommended dilution WB: 1:1000
    ICC/IF: 1:50
    FC: 1:200-1:500
    IP: 1:20-1:50
  • Calculated MW 58 kDa
  • Observed MW 58 kDa
  • Host species Rabbit
  • Clonality Monoclonal
  • Clonality No. DGR34019
  • Isotype IgG
  • Purity Affinity Purification
  • Conjugation Un-conjugated
  • Storage/Stability Store at -20°C. Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA. Stable for 12 months from date of receipt.
  • Western blot analysis of extracts from HeLa, A431 cells and Rat brain tissue using db11072 at 1:1000.
  • Western blot analysis of extracts from A549, HL-60 cells using db11072 at 1:1000.
  • Immunofluorescence analysis of HeLa cells labelling SMARCD1 with db11072.

    The cells were fixed with 4% PFA (10min, RT) followed by treatment with 0.1% Triton X-100 (10min, RT), and blocked in 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween 20 for 1h. The cells were then incubate with db11072 (1:50) at room temprature for 1h, followed by a further incubation at room temperature for 45min with Goat Anti Rabbit lgG (H+L)-AF488 (db10005, shown in green). Nuclear DNA was labeled in blue with DAPI.

    Control: Secondary antibody only.
订购信息
  • Package Price (RMB)
  • 10μL 398
  • 20μL 698
  • 50μL 1398
  • 100μL 2398
  • 货期:现货
For research use only. Not intended for diagnostic.