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Conjugation/Formulation：Un-conjugated
- Background This gene encodes an inhibitor of apoptosis protein (IAP)-binding protein. The encoded mitochondrial protein enters the cytosol when cells undergo apoptosis, and allows activation of caspases by binding to inhibitor of apoptosis proteins. Overexpression of the encoded protein sensitizes tumor cells to apoptosis. A mutation in this gene is associated with young-adult onset of nonsyndromic deafness-64. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, May 2013]
- Immunogen A synthetic peptide of human Smac/Diablo
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Gene ID
56616
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Swiss Prot
Q9NR28
- Synonyms SMAC; DFNA64
- Reactivity Human,Mouse,Rat
- Application WB, IHC-P, ICC/IF, IP
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Recommended dilution
WB: 1:1000
IHC-P: 1:20
ICC/IF: 1:200-1:500
IP: 1:10-1:100 - Calculated MW 27 kDa
- Observed MW 21 kDa
- Host species Rabbit
- Clonality Monoclonal
- Clonality No. DGR12748
- Isotype IgG
- Purity Affinity Purification
- Conjugation Un-conjugated
- Storage/Stability Store at -20°C. Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA. Stable for 12 months from date of receipt.
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Western blot analysis of extracts from K562, HeLa cells using db11117 at 1:1000. -
Immunohistochemical analysis of paraffin-embedded human tonsil using db11117 antibody. -
Western blot analysis of extracts from Mouse heart, Rat skin tissues using db11117 at 1:1000. -
Immunofluorescence analysis of HeLa cells labelling SMAC with db11117.
The cells were fixed with 4% PFA (10min, RT) followed by treatment with 0.1% Triton X-100 (10min, RT), and blocked in 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween 20 for 1h. The cells were then incubate with db11117 (1:200) at room temprature for 1h, followed by a further incubation at room temperature for 45min with Goat Anti Rabbit lgG (H+L)-AF488 (db10005, shown in green). Nuclear DNA was labeled in blue with DAPI.
Control: Secondary antibody only.
订购信息
- Package Price (RMB)
- 10μL 398
- 20μL 698
- 50μL 1398
- 100μL 2398
- 货期：现货
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For research use only. Not intended for diagnostic.
