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Conjugation/Formulation：Un-conjugated
- Background This gene encodes a downstream nuclear effector of the Hippo signaling pathway which is involved in development, growth, repair, and homeostasis. This gene is known to play a role in the development and progression of multiple cancers as a transcriptional regulator of this signaling pathway and may function as a potential target for cancer treatment. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Aug 2013]
- Immunogen A synthetic peptide of human YAP1
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Gene ID
10413
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Swiss Prot
P46937
- Synonyms YAP; YKI; COB1; YAP2; YAP65
- Reactivity Human,Mouse
- Application WB, IHC-P, ICC/IF
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Recommended dilution
WB: 1:1000
IHC-P: 1:200-1:2000
ICC/IF: 1:200-1:500 - Calculated MW 55 kDa
- Observed MW 70-75 kDa
- Host species Rabbit
- Clonality Monoclonal
- Clonality No. DGR14285
- Isotype IgG
- Purity Affinity Purification
- Conjugation Un-conjugated
- Storage/Stability Store at -20°C. Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA. Stable for 12 months from date of receipt.
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Western blot analysis of extracts from HeLa cells using db11127 at 1:1000. -
Immunofluorescence analysis of HeLa cells labelling YAP1 with db11127.
The cells were fixed with 4% PFA (10min, RT) followed by treatment with 0.1% Triton X-100 (10min, RT), and blocked in 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween 20 for 1h. The cells were then incubate with db11127 (1:200) at room temprature for 1h, followed by a further incubation at room temperature for 45min with Goat Anti Rabbit lgG (H+L)-AF488 (db10005, shown in green). Nuclear DNA was labeled in blue with DAPI.
Control: Secondary antibody only.
订购信息
- Package Price (RMB)
- 10μL 398
- 20μL 698
- 50μL 1398
- 100μL 2398
- 货期：现货
相关产品
For research use only. Not intended for diagnostic.
