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Conjugation/Formulation：Un-conjugated
- Background The protein encoded by this gene belongs to the NF-kappa-B inhibitor family, which inhibit NF-kappa-B by complexing with, and trapping it in the cytoplasm. Phosphorylation of serine residues on these proteins by kinases marks them for destruction via the ubiquitination pathway, thereby allowing activation of the NF-kappa-B, which translocates to the nucleus to function as a transcription factor. Alternatively spliced transcript variants have been found for this gene.[provided by RefSeq, Jul 2011]
- Immunogen A synthetic peptide of human IKB beta
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Gene ID
4793
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Swiss Prot
Q15653
- Synonyms IKBB; TRIP9
- Reactivity Human
- Application WB, IHC-P, ICC/IF, FC, IP
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Recommended dilution
WB: 1:1000-1:5000
IHC-P: 1:200-1:500
ICC/IF: 1:50
FC: 1:20-1:50
IP: 1:10-1:100 - Calculated MW 38 kDa
- Observed MW 48 kDa
- Host species Rabbit
- Clonality Monoclonal
- Clonality No. DGR19895
- Isotype IgG
- Purity Affinity Purification
- Conjugation Un-conjugated
- Storage/Stability Store at -20°C. Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA. Stable for 12 months from date of receipt.
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Western blot analysis of extracts from K562, 3T3, HeLa cells using db11257 at 1:1000. -
Western blot analysis of extracts from HCT116 cells using db11257 at 1:1000. -
Immunohistochemical analysis of paraffin-embedded human breast cancer using db11257 antibody. -
Immunofluorescence analysis of MCF-7 cells labelling IKB beta with db11257.
The cells were fixed with 4% PFA (10min, RT) followed by treatment with 0.1% Triton X-100 (10min, RT), and blocked in 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween 20 for 1h. The cells were then incubate with db11257 (1:50) at room temprature for 1h, followed by a further incubation at room temperature for 45min with Goat Anti Rabbit lgG (H+L)-AF488 (db10005, shown in green). Nuclear DNA was labeled in blue with DAPI.
Control: Secondary antibody only.
订购信息
- Package Price (RMB)
- 10μL 398
- 20μL 698
- 50μL 1398
- 100μL 2398
- 货期：现货
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For research use only. Not intended for diagnostic.
