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Conjugation/Formulation：Un-conjugated
- Background Molecular chaperone implicated in a wide variety of cellular processes, including protection of the proteome from stress, folding and transport of newly synthesized polypeptides, activation of proteolysis of misfolded proteins and the formation and dissociation of protein complexes. Plays a pivotal role in the protein quality control system, ensuring the correct folding of proteins, the re-folding of misfolded proteins and controlling the targeting of proteins for subsequent degradation. This is achieved through cycles of ATP binding, ATP hydrolysis and ADP release, mediated by co-chaperones. The co-chaperones have been shown to not only regulate different steps of the ATPase cycle, but they also have an individual specificity such that one co-chaperone may promote folding of a substrate while another may promote degradation. The affinity for polypeptides is regulated by its nucleotide bound state.
- Immunogen Recombinant protein of mouse Hsp70
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Gene ID
15511
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Swiss Prot
P17879
- Synonyms Hsp70; hsp68; Hsp70-1; Hsp70.1
- Reactivity Human,Mouse,Rat
- Application WB, IHC-P, ICC/IF, FC
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Recommended dilution
WB: 1:1000
IHC-P: 1:200-1:1000
ICC/IF: 1:50
FC: 1:100-1:200 - Calculated MW 70 kDa
- Observed MW 70 kDa
- Host species Rabbit
- Clonality Monoclonal
- Clonality No. DGR13507
- Isotype IgG
- Purity Affinity Purification
- Conjugation Un-conjugated
- Storage/Stability Store at -20°C. Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA. Stable for 12 months from date of receipt.
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Western blot analysis of extracts from K562, 3T3, HeLa cells and Rat brain tissue using db11436 at 1:1000. -
Western blot analysis of extracts from Jurkat cells and Rat pancreas, Mouse brain tissues using db11436 at 1:1000. -
Immunohistochemical analysis of paraffin-embedded human tonsil using db11436 antibody. -
Immunofluorescence analysis of 3T3 cells labelling Hsp70 with db11436.
The cells were fixed with cold 100% methanol (10min, 4℃) and blocked in 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween 20 for 1h. The cells were then incubate with db11436 (1:50) at room temprature for 1h, followed by a further incubation at room temperature for 45min with Goat Anti Rabbit lgG (H+L)-AF488 (db10005, shown in green). Nuclear DNA was labeled in blue with DAPI.
Control: Secondary antibody only.
订购信息
- Package Price (RMB)
- 10μL 398
- 20μL 698
- 50μL 1398
- 100μL 2398
- 货期：现货
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For research use only. Not intended for diagnostic.
