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Conjugation/Formulation：Un-conjugated
- Background The Ser/Thr protein kinase encoded by this gene belongs to the CDC5/Polo subfamily. It is highly expressed during mitosis and elevated levels are found in many different types of cancer. Depletion of this protein in cancer cells dramatically inhibited cell proliferation and induced apoptosis; hence, it is a target for cancer therapy. [provided by RefSeq, Sep 2015]
- Immunogen A synthetic peptide of human PLK1
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Gene ID
5347
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Swiss Prot
P53350
- Synonyms PLK; STPK13
- Reactivity Human
- Application WB, ICC/IF, FC, IP
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Recommended dilution
WB: 1:1000
ICC/IF: 1:200-1:500
FC: 1:50-1:100
IP: 1:20-1:50 - Calculated MW 68 kDa
- Observed MW 68 kDa
- Host species Rabbit
- Clonality Monoclonal
- Clonality No. DGR14228
- Isotype IgG
- Purity Affinity Purification
- Conjugation Un-conjugated
- Storage/Stability Store at -20°C. Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA. Stable for 12 months from date of receipt.
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Western blot analysis of extracts from Jurkat, C6 cells using db11470 at 1:1000. -
Western blot analysis of extracts from A549 cells using db11470 at 1:1000. -
Immunofluorescence analysis of HeLa cells labelling PLK1 with db11470.
The cells were fixed with 4% PFA (10min, RT) followed by treatment with 0.1% Triton X-100 (10min, RT), and blocked in 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween 20 for 1h. The cells were then incubate with db11470 (1:200) at room temprature for 1h, followed by a further incubation at room temperature for 45min with Goat Anti Rabbit lgG (H+L)-AF488 (db10005, shown in green). Nuclear DNA was labeled in blue with DAPI.
Control: Secondary antibody only.
订购信息
- Package Price (RMB)
- 10μL 398
- 20μL 698
- 50μL 1398
- 100μL 2398
- 货期：现货
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For research use only. Not intended for diagnostic.
