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Conjugation/Formulation：Un-conjugated
- Background Cyclin B2 is a member of the cyclin family, specifically the B-type cyclins. The B-type cyclins, B1 and B2, associate with p34cdc2 and are essential components of the cell cycle regulatory machinery. B1 and B2 differ in their subcellular localization. Cyclin B1 co-localizes with microtubules, whereas cyclin B2 is primarily associated with the Golgi region. Cyclin B2 also binds to transforming growth factor beta RII and thus cyclin B2/cdc2 may play a key role in transforming growth factor beta-mediated cell cycle control. [provided by RefSeq, Jul 2008]
- Immunogen Recombinant protein of human Cyclin B2/CCNB2
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Gene ID
9133
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Swiss Prot
O95067
- Synonyms HsT17299
- Reactivity Human,Mouse,Rat
- Application WB, IHC-P, ICC/IF, IP
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Recommended dilution
WB: 1:1000
IHC-P: 1:100
ICC/IF: 1:200-1:500
IP: 1:50-1:100 - Calculated MW 45 kDa
- Observed MW 45 kDa
- Host species Rabbit
- Clonality Monoclonal
- Clonality No. DGR31446
- Isotype IgG
- Purity Affinity Purification
- Conjugation Un-conjugated
- Storage/Stability Store at -20°C. Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA. Stable for 12 months from date of receipt.
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Western blot analysis of extracts from HeLa, A549, MCF-7, U251 cells using db11653 at 1:1000. -
Western blot analysis of extracts from HCT116, A431 cells using db11653 at 1:1000. -
Immunofluorescence analysis of HeLa cells labelling Cyclin B2 with db11653.
The cells were fixed with cold 100% methanol (10min, 4℃) and blocked in 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween 20 for 1h. The cells were then incubate with db11653 (1:200) at room temprature for 1h, followed by a further incubation at room temperature for 45min with Goat Anti Rabbit lgG (H+L)-AF488 (db10005, shown in green). Nuclear DNA was labeled in blue with DAPI.
Control: Secondary antibody only.
订购信息
- Package Price (RMB)
- 10μL 398
- 20μL 698
- 50μL 1398
- 100μL 2398
- 货期：现货
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For research use only. Not intended for diagnostic.
