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CaMKII (DGR14655) Rabbit mAb
db11726
Datasheet
- Background The product of this gene belongs to the serine/threonine protein kinases family, and to the Ca(2+)/calmodulin-dependent protein kinases subfamily. Calcium signaling is crucial for several aspects of plasticity at glutamatergic synapses. This calcium calmodulin-dependent protein kinase is composed of four different chains: alpha, beta, gamma, and delta. The alpha chain encoded by this gene is required for hippocampal long-term potentiation (LTP) and spatial learning. In addition to its calcium-calmodulin (CaM)-dependent activity, this protein can undergo autophosphorylation, resulting in CaM-independent activity. Two transcript variants encoding distinct isoforms have been identified for this gene. [provided by RefSeq, Nov 2008]
- Immunogen A synthetic peptide of human CaMKII
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Gene ID
815
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Swiss Prot
Q9UQM7
- Synonyms CAMKA
- Reactivity Human, Mouse, Rat
- Application WB, IHC, ICC/IF, FC
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Recommended dilution
WB: 1:1000-1:2000
IHC: 1:200-1:500
ICC/IF: 1:20-1:100
FC: 1:20-1:100 - Calculated MW 54 kDa
- Observed MW 54 kDa
- Host species Rabbit
- Clonality Monoclonal
- Clonality No. DGR14655
- Isotype IgG
- Purity Affinity Purification
- Conjugation Un-conjugated
- Storage/Stability Store at -20°C. Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA. Stable for 12 months from date of receipt.
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Western blot analysis of extracts from HeLa cells and Rat brain tissue using db11726 at 1:1000. -
Western blot analysis of extracts from HepG2 cells using db11726 at 1:2000. -
Western blot analysis of extracts from 3T3 cells using db11726 at 1:1000. -
Immunofluorescence analysis of HeLa cells labelling CaMKII with db11726.
The cells were fixed with 4% PFA (10min, RT) followed by treatment with 0.1% Triton X-100 (10min, RT), and blocked in 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween 20 for 1h. The cells were then incubate with db11726 (1:20) at room temprature for 1h, followed by a further incubation at room temperature for 45min with Goat Anti Rabbit lgG (H+L)-AF488 (db10005, shown in green). Nuclear DNA was labeled in blue with DAPI.
Control: Secondary antibody only.
订购信息
- Package Price (RMB)
- 10μL 398
- 20μL 698
- 50μL 1398
- 100μL 2398
- 货期：现货
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For research use only. Not intended for diagnostic.
