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Conjugation/Formulation：Un-conjugated
- Background This gene encodes an protein that localizes to the caveolae, which are small domains on the inner cell membranes. This protein plays a role in vesicle trafficking and cell morphology. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jan 2016]
- Immunogen A synthetic peptide of human Flotillin 1
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Gene ID
10211
-
Swiss Prot
O75955
- Synonyms FLOT1
- Reactivity Human,Mouse,Rat
- Application WB, IHC-P, ICC/IF, FC, IP
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Recommended dilution
WB: 1:2000-1:20000
IHC-P: 1:100-1:200
ICC/IF: 1:200-1:500
FC: 1:20-1:50
IP: 1:10-1:100 - Calculated MW 47 kDa
- Observed MW 49 kDa
- Host species Rabbit
- Clonality Monoclonal
- Clonality No. DGR14658
- Isotype IgG
- Purity Affinity Purification
- Conjugation Un-conjugated
- Storage/Stability Store at -20°C. Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA. Stable for 12 months from date of receipt.
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Western blot analysis of extracts from K562, HeLa cells using db11731 at 1:1000. -
Immunofluorescent analysis of HeLa cells using db11731 antibody (green), and DAPI (blue). -
Immunofluorescence analysis of HeLa cells labelling Flotillin 1 with db11731.
The cells were fixed with cold 100% methanol (10min, 4℃) and blocked in 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween 20 for 1h. The cells were then incubate with db11731 (1:200) at room temprature for 1h, followed by a further incubation at room temperature for 45min with Goat Anti Rabbit lgG (H+L)-AF488 (db10005, shown in green). Nuclear DNA was labeled in blue with DAPI.
Control: Secondary antibody only.
订购信息
- Package Price (RMB)
- 10μL 398
- 20μL 698
- 50μL 1398
- 100μL 2398
- 货期：现货
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For research use only. Not intended for diagnostic.
