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Conjugation/Formulation：Un-conjugated
- Background The protein encoded by this gene is a member of the protein tyrosine phosphatase (PTP) family. PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitotic cycle, and oncogenic transformation. This PTP contains two tandem Src homology-2 domains, which function as phospho-tyrosine binding domains and mediate the interaction of this PTP with its substrates. This PTP is widely expressed in most tissues and plays a regulatory role in various cell signaling events that are important for a diversity of cell functions, such as mitogenic activation, metabolic control, transcription regulation, and cell migration. Mutations in this gene are a cause of Noonan syndrome as well as acute myeloid leukemia. [provided by RefSeq, Aug 2016]
- Immunogen A synthetic peptide of human SHP2
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Gene ID
5781
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Swiss Prot
Q06124
- Synonyms CFC; NS1; JMML; SHP2; BPTP3; PTP2C; METCDS; PTP-1D; SH-PTP2; SH-PTP3
- Reactivity Human
- Application WB, IHC-P, ICC/IF, FC, IP
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Recommended dilution
WB: 1:1000-1:5000
IHC-P: 1:100
ICC/IF: 1:50
FC: 1:20-1:50
IP: 1:20-1:50 - Calculated MW 68 kDa
- Observed MW 68 kDa
- Host species Rabbit
- Clonality Monoclonal
- Clonality No. DGR11556
- Isotype IgG
- Purity Affinity Purification
- Conjugation Un-conjugated
- Storage/Stability Store at -20°C. Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA. Stable for 12 months from date of receipt.
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Western blot analysis of extracts from HeLa, 293 cells using db11960 at 1:1000. -
Western blot analysis of extracts from HCT116 cells using db11960 at 1:2000. -
Immunohistochemical analysis of paraffin-embedded human breast cancer using db11960 antibody. -
Immunofluorescence analysis of A431 cells labelling SHP2 with db11960.
The cells were fixed with 4% PFA (10min, RT) followed by treatment with 0.1% Triton X-100 (10min, RT), and blocked in 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween 20 for 1h. The cells were then incubate with db11960 (1:50) at room temprature for 1h, followed by a further incubation at room temperature for 45min with Goat Anti Rabbit lgG (H+L)-AF488 (db10005, shown in green). Nuclear DNA was labeled in blue with DAPI.
Control: Secondary antibody only.
订购信息
- Package Price (RMB)
- 10μL 398
- 20μL 698
- 50μL 1398
- 100μL 2398
- 货期：现货
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For research use only. Not intended for diagnostic.
