您当前的位置：
Conjugation/Formulation：Un-conjugated
- Background The protein encoded by this gene is one of the three catalytic subunits of protein phosphatase 1 (PP1). PP1 is a serine/threonine specific protein phosphatase known to be involved in the regulation of a variety of cellular processes, such as cell division, glycogen metabolism, muscle contractility, protein synthesis, and HIV-1 viral transcription. Increased PP1 activity has been observed in the end stage of heart failure. Studies in both human and mice suggest that PP1 is an important regulator of cardiac function. Mouse studies also suggest that PP1 functions as a suppressor of learning and memory. Three alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
- Immunogen A synthetic peptide of human PPP1CA+PPP1CB
-
Gene ID
5499
-
Swiss Prot
P62136
- Synonyms PP1A; PP-1A; PPP1A; PP1alpha
- Reactivity Human,Mouse,Rat
- Application WB, IHC-P, ICC/IF, FC, IP
-
Recommended dilution
WB: 1:5000-1:50000
IHC-P: 1:50-1:100
ICC/IF: 1:50-1:200
FC: 1:100-1:200
IP: 1:20-1:100 - Calculated MW 38 kDa
- Observed MW 38 kDa
- Host species Rabbit
- Clonality Monoclonal
- Clonality No. DGR33032
- Isotype IgG
- Purity Affinity Purification
- Conjugation Un-conjugated
- Storage/Stability Store at -20°C. Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA. Stable for 12 months from date of receipt.
×
![]()
-
Western blot analysis of extracts from K562 cells and Rat brain tissue using db12099 at 1:1000. -
Immunohistochemical analysis of paraffin-embedded mouse colon using db12099 antibody. -
Western blot analysis of extracts from RAW264.7 cells and Mouse kidney tissue using db12099 at 1:1000. -
Western blot analysis of extracts from HepG2, HCT116 cells using db12099 at 1:1000. -
Immunofluorescence analysis of HeLa cells labelling PP1C alpha/beta with db12099.
The cells were fixed with 4% PFA (10min, RT) followed by treatment with 0.1% Triton X-100 (10min, RT), and blocked in 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween 20 for 1h. The cells were then incubate with db12099 (1:50) at room temprature for 1h, followed by a further incubation at room temperature for 45min with Goat Anti Rabbit lgG (H+L)-AF488 (db10005, shown in green). Nuclear DNA was labeled in blue with DAPI.
Control: Secondary antibody only.
订购信息
- Package Price (RMB)
- 10μL 398
- 20μL 698
- 50μL 1398
- 100μL 2398
- 货期：现货
相关产品
For research use only. Not intended for diagnostic.
