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Conjugation/Formulation：Un-conjugated
- Background The protein encoded by this gene is a member of the CMGC family of serine/threonine protein kinases. This kinase is a catalytic subunit of the protein kinase complex that is important for cell cycle G1 phase progression and G1/S transition. The activity of this kinase first appears in mid-G1 phase, which is controlled by the regulatory subunits including D-type cyclins and members of INK4 family of CDK inhibitors. This kinase, as well as CDK4, has been shown to phosphorylate, and thus regulate the activity of, tumor suppressor protein Rb. Altered expression of this gene has been observed in multiple human cancers. A mutation in this gene resulting in reduced cell proliferation, and impaired cell motility and polarity, and has been identified in patients with primary microcephaly. [provided by RefSeq, Aug 2017]
- Immunogen A synthetic peptide of human Cdk6
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Gene ID
1021
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Swiss Prot
Q00534
- Synonyms MCPH12; PLSTIRE
- Reactivity Human
- Application WB, IHC-P, ICC/IF, FC
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Recommended dilution
WB: 1:5000-1:50000
IHC-P: 1:100-1:200
ICC/IF: 1:200-1:500
FC: 1:100-1:1000 - Calculated MW 37 kDa
- Observed MW 37 kDa
- Host species Rabbit
- Clonality Monoclonal
- Clonality No. DGR11918
- Isotype IgG
- Purity Affinity Purification
- Conjugation Un-conjugated
- Storage/Stability Store at -20°C. Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA. Stable for 12 months from date of receipt.
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Western blot analysis of extracts from K562,HeLa cells using db12115 at 1:1000. -
Immunofluorescence analysis of HeLa cells labelling CDK6 with db12115.
The cells were fixed with 4% PFA (10min, RT) followed by treatment with 0.1% Triton X-100 (10min, RT), and blocked in 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween 20 for 1h. The cells were then incubate with db12115 (1:200) at room temprature for 1h, followed by a further incubation at room temperature for 45min with Goat Anti Rabbit lgG (H+L)-AF488 (db10005, shown in green). Nuclear DNA was labeled in blue with DAPI.
Control: Secondary antibody only.
订购信息
- Package Price (RMB)
- 10μL 398
- 20μL 698
- 50μL 1398
- 100μL 2398
- 货期：现货
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For research use only. Not intended for diagnostic.
