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Conjugation/Formulation：Un-conjugated
- Background Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.
- Immunogen A synthetic methylpeptide corresponding to residues surrounding Lys56 of human Histone H3
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Gene ID
8350
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Swiss Prot
P68431
- Synonyms H3/A; H3FA
- Reactivity Human,Mouse
- Application WB, ICC/IF
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Recommended dilution
WB: 1:1000
ICC/IF: 1:100 - Calculated MW 15 kDa
- Observed MW 17 kDa
- Host species Rabbit
- Clonality Monoclonal
- Clonality No. DGR33460
- Isotype IgG
- Purity Affinity Purification
- Conjugation Un-conjugated
- Storage/Stability Store at -20°C. Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA. Stable for 12 months from date of receipt.
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Western blot analysis of extracts from Mouse heart tissue using db12216 at 1:1000. -
Immunofluorescence analysis of HeLa cells labelling DiMethyl-Histone H3 (Lys56) with db12216.
The cells were fixed with 4% PFA (10min, RT) followed by treatment with 0.1% Triton X-100 (10min, RT), and blocked in 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween 20 for 1h. The cells were then incubate with db12216 (1:100) at room temprature for 1h, followed by a further incubation at room temperature for 45min with Goat Anti Rabbit lgG (H+L)-AF488 (db10005, shown in green). Nuclear DNA was labeled in blue with DAPI.
Control: Secondary antibody only.
订购信息
- Package Price (RMB)
- 10μL 398
- 20μL 698
- 50μL 1398
- 100μL 2398
- 货期：现货
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For research use only. Not intended for diagnostic.
