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Conjugation/Formulation：Un-conjugated
- Background This gene encodes a member of the class-I pyridine nucleotide-disulfide oxidoreductase family. The encoded protein has been identified as a moonlighting protein based on its ability to perform mechanistically distinct functions. In homodimeric form, the encoded protein functions as a dehydrogenase and is found in several multi-enzyme complexes that regulate energy metabolism. However, as a monomer, this protein can function as a protease. Mutations in this gene have been identified in patients with E3-deficient maple syrup urine disease and lipoamide dehydrogenase deficiency. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jan 2014]
- Immunogen A synthetic peptide of human Lipoamide Dehydrogenase
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Gene ID
1738
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Swiss Prot
P09622
- Synonyms E3; LAD; DLDD; DLDH; GCSL; PHE3
- Reactivity Human,Mouse,Rat
- Application WB, IHC-P, ICC/IF
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Recommended dilution
WB: 1:2000-1:20000
IHC-P: 1:100-1:200
ICC/IF: 1:100-1:200 - Calculated MW 54 kDa
- Observed MW 54 kDa
- Host species Rabbit
- Clonality Monoclonal
- Clonality No. DGR11788
- Isotype IgG
- Purity Affinity Purification
- Conjugation Un-conjugated
- Storage/Stability Store at -20°C. Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA. Stable for 12 months from date of receipt.
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Western blot analysis of extracts from K562, C6, 3T3, HeLa cells and Rat brain tissue using db12224 at 1:1000. -
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue using db12224 antibody. -
Western blot analysis of extracts from 293T, SH-SY5Y cells and Mouse heart tissue using db12224 at 1:1000. -
Immunofluorescence analysis of HeLa cells labelling Lipoamide Dehydrogenase with db12224.
The cells were fixed with 4% PFA (10min, RT) followed by treatment with 0.1% Triton X-100 (10min, RT), and blocked in 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween 20 for 1h. The cells were then incubate with db12224 (1:100) at room temprature for 1h, followed by a further incubation at room temperature for 45min with Goat Anti Rabbit lgG (H+L)-AF488 (db10005, shown in green). Nuclear DNA was labeled in blue with DAPI.
Control: Secondary antibody only.
订购信息
- Package Price (RMB)
- 10μL 398
- 20μL 698
- 50μL 1398
- 100μL 2398
- 货期：现货
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For research use only. Not intended for diagnostic.
