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Conjugation/Formulation：Un-conjugated
- Background This gene belongs to the protein kinase superfamily. The encoded protein contains a protein kinase-like domain; however, is thought to be inactive because it lacks several residues required for activity. This protein plays a critical role in tumor necrosis factor (TNF)-induced necroptosis, a programmed cell death process, via interaction with receptor-interacting protein 3 (RIP3), which is a key signaling molecule in necroptosis pathway. Inhibitor studies and knockdown of this gene inhibited TNF-induced necrosis. High levels of this protein and RIP3 are associated with inflammatory bowel disease in children. Alternatively spliced transcript variants have been described for this gene. [provided by RefSeq, Sep 2015]
- Immunogen Recombinant protein of human MLKL
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Gene ID
197259
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Swiss Prot
Q8NB16
- Synonyms hMLKL
- Reactivity Human
- Application WB, IHC-P, ICC/IF
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Recommended dilution
WB: 1:1000
IHC-P: 1:100-1:200
ICC/IF: 1:100-1:200 - Calculated MW 55 kDa
- Observed MW 55 kDa
- Host species Rabbit
- Clonality Monoclonal
- Clonality No. DGR12141
- Isotype IgG
- Purity Affinity Purification
- Conjugation Un-conjugated
- Storage/Stability Store at -20°C. Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA. Stable for 12 months from date of receipt.
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Western blot analysis of extracts from U251, HeLa cells using db12457 at 1:1000. -
Western blot analysis of extracts from HT-29 cells using db12457 at 1:1000. -
Immunofluorescence analysis of HeLa cells labelling MLKL with db12457.
The cells were fixed with cold 100% methanol (10min, 4℃) and blocked in 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween 20 for 1h. The cells were then incubate with db12457 (1:100) at room temprature for 1h, followed by a further incubation at room temperature for 45min with Goat Anti Rabbit lgG (H+L)-AF488 (db10005, shown in green). Nuclear DNA was labeled in blue with DAPI.
Control: Secondary antibody only.
订购信息
- Package Price (RMB)
- 10μL 398
- 20μL 698
- 50μL 1398
- 100μL 2398
- 货期：现货
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For research use only. Not intended for diagnostic.
