HIF-1-alpha单克隆抗体(DGR11600) | 高特异性&高亲和力

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Recombinant DGRmAb^®

HIF-1-alpha (DGR11600) Rabbit mAb

db12576

暑期特惠 10μl特价

Datasheet

Product Name : HIF-1-alpha (DGR11600) Rabbit mAb

Cat.No.: db12576

Synonyms : HIF1; MOP1; PASD8; HIF-1A; bHLHe78; HIF-1alpha; HIF1-ALPHA; HIF-1-alpha

Application：

Reactivity : Human

Host species : Rabbit

Un-conjugated PBS Only

Background This gene encodes the alpha subunit of transcription factor hypoxia-inducible factor-1 (HIF-1), which is a heterodimer composed of an alpha and a beta subunit. HIF-1 functions as a master regulator of cellular and systemic homeostatic response to hypoxia by activating transcription of many genes, including those involved in energy metabolism, angiogenesis, apoptosis, and other genes whose protein products increase oxygen delivery or facilitate metabolic adaptation to hypoxia. HIF-1 thus plays an essential role in embryonic vascularization, tumor angiogenesis and pathophysiology of ischemic disease. Alternatively spliced transcript variants encoding different isoforms have been identified for this gene. [provided by RefSeq, Jul 2011]
Immunogen A synthetic peptide of human HIF-1-alpha
Gene ID 3091
Swiss Prot Q16665
Synonyms HIF1; MOP1; PASD8; HIF-1A; bHLHe78; HIF-1alpha; HIF1-ALPHA; HIF-1-alpha
Reactivity Human
Application WB, IHC, ICC/IF, FC, IP
Recommended dilution WB: 1:1000
IHC: 1:100
ICC/IF: 1:200-1:500
FC: 1:500-1:2000
IP: 1:50-1:200
Calculated MW 93 kDa
Observed MW 120 kDa
Host species Rabbit
Clonality Monoclonal
Clonality No. DGR11600
Isotype IgG
Purity Affinity Purification
Conjugation Un-conjugated
Storage/Stability Store at -20°C. Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA. Stable for 12 months from date of receipt.

Immunofluorescence analysis of HeLa cells labelling HIF-1-alpha with db12576.

The cells were fixed with 4% PFA (10min, RT) followed by treatment with 0.1% Triton X-100 (10min, RT), and blocked in 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween 20 for 1h. The cells were then incubate with db12576 (1:200) at room temprature for 1h, followed by a further incubation at room temperature for 45min with Goat Anti Rabbit lgG (H+L)-AF488 (db10005, shown in green). Nuclear DNA was labeled in blue with DAPI.

Control: Secondary antibody only.

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