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Conjugation/Formulation：Un-conjugated
- Background This gene is the cellular homolog of viral raf gene (v-raf). The encoded protein is a MAP kinase kinase kinase (MAP3K), which functions downstream of the Ras family of membrane associated GTPases to which it binds directly. Once activated, the cellular RAF1 protein can phosphorylate to activate the dual specificity protein kinases MEK1 and MEK2, which in turn phosphorylate to activate the serine/threonine specific protein kinases, ERK1 and ERK2. Activated ERKs are pleiotropic effectors of cell physiology and play an important role in the control of gene expression involved in the cell division cycle, apoptosis, cell differentiation and cell migration. Mutations in this gene are associated with Noonan syndrome 5 and LEOPARD syndrome 2. [provided by RefSeq, Jul 2008]
- Immunogen A synthetic peptide of human Raf1
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Gene ID
5894
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Swiss Prot
P04049
- Synonyms NS5; CRAF; Raf-1; c-Raf; CMD1NN
- Reactivity Human,Mouse,Rat
- Application WB, ICC/IF, FC
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Recommended dilution
WB: 1:1000
ICC/IF: 1:100-1:200
FC: 1:100 - Calculated MW 73 kDa
- Observed MW 73 kDa
- Host species Rabbit
- Clonality Monoclonal
- Clonality No. DGR15751
- Isotype IgG
- Purity Affinity Purification
- Conjugation Un-conjugated
- Storage/Stability Store at -20°C. Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA. Stable for 12 months from date of receipt.
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Western blot analysis of extracts from C6, 3T3, HeLa cells using db12651 at 1:1000. -
Immunofluorescent analysis of MCF-7 cells using db12651 antibody (green), and DAPI (blue). -
Immunofluorescence analysis of HeLa cells labelling Raf1 with db12651.
The cells were fixed with 4% PFA (10min, RT) followed by treatment with 0.1% Triton X-100 (10min, RT), and blocked in 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween 20 for 1h. The cells were then incubate with db12651 (1:100) at room temprature for 1h, followed by a further incubation at room temperature for 45min with Goat Anti Rabbit lgG (H+L)-AF488 (db10005, shown in green). Nuclear DNA was labeled in blue with DAPI.
Control: Secondary antibody only.
订购信息
- Package Price (RMB)
- 10μL 398
- 20μL 698
- 50μL 1398
- 100μL 2398
- 货期：现货
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For research use only. Not intended for diagnostic.
