您当前的位置：
Conjugation/Formulation：Un-conjugated
- Background This gene encodes a member of the RNA-binding FOX protein family which is involved in the regulation of alternative splicing of pre-mRNA. The protein has an N-terminal proline-rich region, an RNA recognition motif (RRM) domain, and a C-terminal alanine-rich region. This gene produces the neuronal nuclei (NeuN) antigen that has been widely used as a marker for post-mitotic neurons. This gene has its highest expression in the central nervous system and plays a prominent role in neural tissue development and regulation of adult brain function. Mutations in this gene have been associated with numerous neurological disorders. Alternative splicing of this gene results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq, May 2017]
- Immunogen A synthetic peptide of human NeuN
-
Gene ID
146713
-
Swiss Prot
A6NFN3
- Synonyms FOX3; NEUN; FOX-3; HRNBP3
- Reactivity Human,Mouse,Rat
- Application WB, IHC-P, ICC/IF, FC
-
Recommended dilution
WB: 1:1000-1:5000
IHC-P: 1:500-1:2000
ICC/IF: 1:100-1:200
FC: 1:100 - Calculated MW 34 kDa
- Observed MW 46-55 kDa
- Host species Rabbit
- Clonality Monoclonal
- Clonality No. DGR13944
- Isotype IgG
- Purity Affinity Purification
- Conjugation Un-conjugated
- Storage/Stability Store at -20°C. Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA. Stable for 12 months from date of receipt.
×
![]()
-
Western blot analysis of extracts from Rat brain tissue using db12692 at 1:1000. -
Immunofluorescent analysis of SH-SY5Y cells using db12692 antibody (green), and DAPI (blue). -
Immunofluorescence analysis of A549 cells labelling NeuN with db12692.
The cells were fixed with 4% PFA (10min, RT) followed by treatment with 0.1% Triton X-100 (10min, RT), and blocked in 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween 20 for 1h. The cells were then incubate with db12692 (1:100) at room temprature for 1h, followed by a further incubation at room temperature for 45min with Goat Anti Rabbit lgG (H+L)-AF488 (db10005, shown in green). Nuclear DNA was labeled in blue with DAPI.
Control: Secondary antibody only. -
Immunohistochemical analysis of paraffin-embedded human glioblastoma tissue labeling NeuN with db12692. Counter stained with Hematoxylin. -
Immunohistochemical analysis of paraffin-embedded human kidney tissue labeling NeuN with db12692. Counter stained with Hematoxylin.
订购信息
- Package Price (RMB)
- 10μL 398
- 20μL 698
- 50μL 1398
- 100μL 2398
- 货期：现货
相关产品
For research use only. Not intended for diagnostic.
