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Conjugation/Formulation：Un-conjugated
- Background The protein encoded by this gene is part of the multimeric HBO1 complex, which possesses histone H4-specific acetyltransferase activity. This activity is required for functional replication origins and is involved in transcriptional activation of some genes. In both cases, the acetylation of histone H4 helps unfold chromatin so that the DNA can be accessed and replicated or transcribed. [provided by RefSeq, Oct 2016]
- Immunogen Recombinant protein of human KAT7/Hbo1/MYST2
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Gene ID
11143
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Swiss Prot
O95251
- Synonyms HBO1; HBOA; MYST2; ZC2HC7
- Reactivity Human,Mouse,Rat
- Application WB, IHC-P, ICC/IF, IP
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Recommended dilution
WB: 1:1000-1:5000
IHC-P: 1:100-1:200
ICC/IF: 1:500-1:2000
IP: 1:50 - Calculated MW 71 kDa
- Observed MW 71 kDa
- Host species Rabbit
- Clonality Monoclonal
- Clonality No. DGR32553
- Isotype IgG
- Purity Affinity Purification
- Conjugation Un-conjugated
- Storage/Stability Store at -20°C. Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA. Stable for 12 months from date of receipt.
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Western blot analysis of extracts from HeLa, A549, 293T cells using db13014 at 1:1000. -
Immunohistochemical analysis of paraffin-embedded human lung cancer tissue using db13014 antibody. -
Immunofluorescent analysis of HCT116 cells using db13014 antibody (green), and DAPI (blue). -
Immunofluorescence analysis of HeLa cells labelling KAT7 with db13014.
The cells were fixed with 4% PFA (10min, RT) followed by treatment with 0.1% Triton X-100 (10min, RT), and blocked in 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween 20 for 1h. The cells were then incubate with db13014 (1:500) at room temprature for 1h, followed by a further incubation at room temperature for 45min with Goat Anti Rabbit lgG (H+L)-AF488 (db10005, shown in green). Nuclear DNA was labeled in blue with DAPI.
Control: Secondary antibody only.
订购信息
- Package Price (RMB)
- 10μL 398
- 20μL 698
- 50μL 1398
- 100μL 2398
- 货期：现货
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For research use only. Not intended for diagnostic.
