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Conjugation/Formulation：Un-conjugated
- Background This gene belongs to the protein arginine methyltransferase (PRMT) family. The encoded enzyme catalyzes the methylation of guanidino nitrogens of arginyl residues of proteins. The enzyme acts on 40S ribosomal protein S2 (rpS2), which is its major in-vivo substrate, and is involved in the proper maturation of the 80S ribosome. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Aug 2013]
- Immunogen A synthetic peptide of human PRMT3
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Gene ID
10196
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Swiss Prot
O60678
- Synonyms HRMT1L3
- Reactivity Human
- Application WB, IHC-P, ICC/IF, IP
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Recommended dilution
WB: 1:2000-1:20000
IHC-P: 1:100
ICC/IF: 1:50
IP: 1:20-1:50 - Calculated MW 60 kDa
- Observed MW 60 kDa
- Host species Rabbit
- Clonality Monoclonal
- Clonality No. DGR16719
- Isotype IgG
- Purity Affinity Purification
- Conjugation Un-conjugated
- Storage/Stability Store at -20°C. Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA. Stable for 12 months from date of receipt.
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Western blot analysis of extracts from C6, 3T3, HeLa cells and Rat brain tissue using db13113 at 1:1000. -
Western blot analysis of extracts from T24 cells using db13113 at 1:6000. -
Immunohistochemical analysis of paraffin-embedded human lung cancer using db13113 antibody. -
Immunofluorescence analysis of HeLa cells labelling PRMT3 with db13113.
The cells were fixed with cold 100% methanol (10min, 4℃) and blocked in 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween 20 for 1h. The cells were then incubate with db13113 (1:50) at room temprature for 1h, followed by a further incubation at room temperature for 45min with Goat Anti Rabbit lgG (H+L)-AF488 (db10005, shown in green). Nuclear DNA was labeled in blue with DAPI.
Control: Secondary antibody only.
订购信息
- Package Price (RMB)
- 10μL 398
- 20μL 698
- 50μL 1398
- 100μL 2398
- 货期：现货
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For research use only. Not intended for diagnostic.
