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Conjugation/Formulation：Un-conjugated
- Background The protein encoded by this gene, in combination with autophagy protein 12, functions as an E1-like activating enzyme in a ubiquitin-like conjugating system. The encoded protein is involved in several cellular processes, including autophagic vesicle formation, mitochondrial quality control after oxidative damage, negative regulation of the innate antiviral immune response, lymphocyte development and proliferation, MHC II antigen presentation, adipocyte differentiation, and apoptosis. Several transcript variants encoding different protein isoforms have been found for this gene. [provided by RefSeq, Sep 2015]
- Immunogen A synthetic peptide of human APG5L/ATG5
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Gene ID
9474
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Swiss Prot
Q9H1Y0
- Synonyms ASP; APG5; APG5L; hAPG5; SCAR25; APG5-LIKE
- Reactivity Human,Mouse,Rat
- Application WB, IHC-P, ICC/IF, IP
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Recommended dilution
WB: 1:1000-1:5000
IHC-P: 1:100-1:200
ICC/IF: 1:100-1:200
IP: 1:10-1:100 - Calculated MW 33 kDa
- Observed MW 55 kDa
- Host species Rabbit
- Clonality Monoclonal
- Clonality No. DGR15109
- Isotype IgG
- Purity Affinity Purification
- Conjugation Un-conjugated
- Storage/Stability Store at -20°C. Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA. Stable for 12 months from date of receipt.
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Western blot analysis of extracts from Rat brain tissue using db13177 at 1:1000. -
Western blot analysis of extracts from K562, C6, HeLa cells using db13177 at 1:1000. -
Immunofluorescent analysis of MCF-7 cells using db13177 antibody (green), and DAPI (blue). -
Western blot analysis of extracts from RAW264.7 cells using db13177 at 1:1000. -
Immunofluorescence analysis of HeLa cells labelling APG5L/ATG5 with db13177.
The cells were fixed with cold 100% methanol (10min, 4℃) and blocked in 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween 20 for 1h. The cells were then incubate with db13177 (1:100) at room temprature for 1h, followed by a further incubation at room temperature for 45min with Goat Anti Rabbit lgG (H+L)-AF488 (db10005, shown in green). Nuclear DNA was labeled in blue with DAPI.
Control: Secondary antibody only.
订购信息
- Package Price (RMB)
- 10μL 398
- 20μL 698
- 50μL 1398
- 100μL 2398
- 货期：现货
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For research use only. Not intended for diagnostic.
