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Conjugation/Formulation：Un-conjugated
- Background The protein encoded by this gene belongs to the histone deacetylase family, members of which deacetylate lysine residues on the N-terminal part of the core histones. Histone deacetylation modulates chromatin structure, and plays an important role in transcriptional regulation, cell cycle progression, and developmental events. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Aug 2011]
- Immunogen A synthetic peptide of human HDAC10
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Gene ID
83933
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Swiss Prot
Q969S8
- Synonyms HD10
- Reactivity Human
- Application WB, ICC/IF, FC, IP
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Recommended dilution
WB: 1:1000
ICC/IF: 1:100-1:200
FC: 1:100-1:200
IP: 1:100 - Calculated MW 72 kDa
- Observed MW 72 kDa
- Host species Rabbit
- Clonality Monoclonal
- Clonality No. DGR32951
- Isotype IgG
- Purity Affinity Purification
- Conjugation Un-conjugated
- Storage/Stability Store at -20°C. Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA. Stable for 12 months from date of receipt.
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Western blot analysis of extracts from 293T, HepG2 cells using db13327 at 1:1000. -
Immunofluorescence analysis of HeLa cells labelling HDAC10 with db13327.
The cells were fixed with cold 100% methanol (10min, 4℃) and blocked in 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween 20 for 1h. The cells were then incubate with db13327 (1:100) at room temprature for 1h, followed by a further incubation at room temperature for 45min with Goat Anti Rabbit lgG (H+L)-AF488 (db10005, shown in green). Nuclear DNA was labeled in blue with DAPI.
Control: Secondary antibody only.
订购信息
- Package Price (RMB)
- 10μL 398
- 20μL 698
- 50μL 1398
- 100μL 2398
- 货期：现货
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For research use only. Not intended for diagnostic.
