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Conjugation/Formulation：Un-conjugated
- Background Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a replication-independent histone that is a member of the histone H1 family. [provided by RefSeq, Oct 2015]
- Immunogen A synthetic peptide of human Histone H1.0
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Gene ID
3005
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Swiss Prot
P07305
- Synonyms H10; H1FV
- Reactivity Human,Mouse,Rat
- Application WB, IHC-P, ICC/IF
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Recommended dilution
WB: 1:1000-1:5000
IHC-P: 1:100-1:500
ICC/IF: 1:100-1:200 - Calculated MW 21 kDa
- Observed MW 30 kDa
- Host species Rabbit
- Clonality Monoclonal
- Clonality No. DGR11559
- Isotype IgG
- Purity Affinity Purification
- Conjugation Un-conjugated
- Storage/Stability Store at -20°C. Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA. Stable for 12 months from date of receipt.
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Western blot analysis of extracts from HeLa cells using db13453 at 1:1000. -
Immunofluorescence analysis of A431 cells labelling Histone H1.0 with db13453.
The cells were fixed with 4% PFA (10min, RT) followed by treatment with 0.1% Triton X-100 (10min, RT), and blocked in 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween 20 for 1h. The cells were then incubate with db13453 (1:100) at room temprature for 1h, followed by a further incubation at room temperature for 45min with Goat Anti Rabbit lgG (H+L)-AF488 (db10005, shown in green). Nuclear DNA was labeled in blue with DAPI.
Control: Secondary antibody only.
订购信息
- Package Price (RMB)
- 10μL 398
- 20μL 698
- 50μL 1398
- 100μL 2398
- 货期：现货
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For research use only. Not intended for diagnostic.
