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Conjugation/Formulation：Un-conjugated
- Background Protein kinase C (PKC) is a family of serine- and threonine-specific protein kinases that can be activated by calcium and second messenger diacylglycerol. PKC family members phosphorylate a wide variety of protein targets and are known to be involved in diverse cellular signaling pathways. PKC family members also serve as major receptors for phorbol esters, a class of tumor promoters. Each member of the PKC family has a specific expression profile and is believed to play a distinct role in cells. The protein encoded by this gene is one of the PKC family members. This protein kinase has been reported to be involved in many different cellular functions, such as B cell activation, apoptosis induction, endothelial cell proliferation, and intestinal sugar absorption. Studies in mice also suggest that this kinase may also regulate neuronal functions and correlate fear-induced conflict behavior after stress. Alternatively spliced transcript variants encoding distinct isoforms have been reported. [provided by RefSeq, Jul 2008]
- Immunogen A synthetic peptide of human PKC beta 2
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Gene ID
5579
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Swiss Prot
P05771
- Synonyms PKCB; PRKCB1; PRKCB2; PKC-beta
- Reactivity Human, Rat
- Application WB, IHC, ICC/IF, FC, IP
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Recommended dilution
WB: 1:1000
IHC: 1:20
ICC/IF: 1:50
FC: 1:20
IP: 1:20 - Calculated MW 77 kDa
- Observed MW 77 kDa
- Host species Rabbit
- Clonality Monoclonal
- Clonality No. DGR19556
- Isotype IgG
- Purity Affinity Purification
- Conjugation Un-conjugated
- Storage/Stability Store at -20°C. Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA. Stable for 12 months from date of receipt.
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Western blot analysis of extracts from K562 cells using db13454 at 1:1000. -
Western blot analysis of extracts from Rat brain tissue using db13454 at 1:1000. -
Immunofluorescence analysis of K562 cells labelling PKC beta 2 with db13454.
The cells were fixed with cold 100% methanol (10min, 4℃) and blocked in 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween 20 for 1h. The cells were then incubate with db13454 (1:50) at room temprature for 1h, followed by a further incubation at room temperature for 45min with Goat Anti Rabbit lgG (H+L)-AF488 (db10005, shown in green). Nuclear DNA was labeled in blue with DAPI.
Control: Secondary antibody only.
订购信息
- Package Price (RMB)
- 10μL 398
- 20μL 698
- 50μL 1398
- 100μL 2398
- 货期：现货
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For research use only. Not intended for diagnostic.
