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beta Catenin (DGR12883) Rabbit mAb
db13925
Datasheet
- Background The protein encoded by this gene is part of a complex of proteins that constitute adherens junctions (AJs). AJs are necessary for the creation and maintenance of epithelial cell layers by regulating cell growth and adhesion between cells. The encoded protein also anchors the actin cytoskeleton and may be responsible for transmitting the contact inhibition signal that causes cells to stop dividing once the epithelial sheet is complete. Finally, this protein binds to the product of the APC gene, which is mutated in adenomatous polyposis of the colon. Mutations in this gene are a cause of colorectal cancer (CRC), pilomatrixoma (PTR), medulloblastoma (MDB), and ovarian cancer. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Aug 2016]
- Immunogen A synthetic peptide of human beta Catenin
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Gene ID
1499
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Swiss Prot
P35222
- Synonyms EVR7; CTNNB; MRD19; armadillo
- Reactivity Human,Mouse,Rat
- Application WB, IHC-P, ICC/IF, IP
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Recommended dilution
WB: 1:1000-1:5000
IHC-P: 1:200-1:500
ICC/IF: 1:100-1:200
IP: 1:20-1:50 - Calculated MW 86 kDa
- Observed MW 86 kDa
- Host species Rabbit
- Clonality Monoclonal
- Clonality No. DGR12883
- Isotype IgG
- Purity Affinity Purification
- Conjugation Un-conjugated
- Storage/Stability Store at -20°C. Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA. Stable for 12 months from date of receipt.
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Western blot analysis of extracts from C6,CHO-KI,HeLa cells using db13925 at 1:1000. -
Immunohistochemical analysis of paraffin-embedded human Cholangiocarcinoma using db13925 antibody. -
Western blot analysis of extracts from Mouse spleen tissue using db13925 at 1:1000. -
Immunofluorescence analysis of HeLa cells labelling beta Catenin with db13925.
The cells were fixed with cold 100% methanol (10min, 4℃) and blocked in 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween 20 for 1h. The cells were then incubate with db13925 (1:100) at room temprature for 1h, followed by a further incubation at room temperature for 45min with Goat Anti Rabbit lgG (H+L)-AF488 (db10005, shown in green). Nuclear DNA was labeled in blue with DAPI.
Control: Secondary antibody only.
订购信息
- Package Price (RMB)
- 10μL 398
- 20μL 698
- 50μL 1398
- 100μL 2398
- 货期：现货
相关产品
For research use only. Not intended for diagnostic.
