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Conjugation/Formulation：Un-conjugated
- Background This gene encodes a cell surface receptor necessary for cellular iron uptake by the process of receptor-mediated endocytosis. This receptor is required for erythropoiesis and neurologic development. Multiple alternatively spliced variants have been identified. [provided by RefSeq, Sep 2015]
- Immunogen Recombinant protein of human Transferrin Receptor
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Gene ID
7037
-
Swiss Prot
P02786
- Synonyms T9; TR; TFR; p90; CD71; TFR1; TRFR; IMD46
- Reactivity Human,Mouse,Rat
- Application WB, IHC-P, ICC/IF, IP
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Recommended dilution
WB: 1:1000
IHC-P: 1:200-1:500
ICC/IF: 1:100
IP: 1:20-1:50 - Calculated MW 84 kDa
- Observed MW 90 kDa
- Host species Rabbit
- Clonality Monoclonal
- Clonality No. DGR14579
- Isotype IgG
- Purity Affinity Purification
- Conjugation Un-conjugated
- Storage/Stability Store at -20°C. Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA. Stable for 12 months from date of receipt.
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Western blot analysis of extracts from K562 and HeLa cells using db14195 at 1:1000. -
Immunofluorescence analysis of HeLa cells labelling Transferrin Receptor with db14195.
The cells were fixed with cold 100% methanol (10min, 4℃) and blocked in 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween 20 for 1h. The cells were then incubate with db14195 (1:100) at room temprature for 1h, followed by a further incubation at room temperature for 45min with Goat Anti Rabbit lgG (H+L)-AF488 (db10005, shown in green). Nuclear DNA was labeled in blue with DAPI.
Control: Secondary antibody only. -
Immunohistochemical analysis of paraffin-embedded human liver carcinoma tissue labeling Transferrin Receptor with db14195. Counter stained with Hematoxylin. -
Immunohistochemical analysis of paraffin-embedded human placental tissue labeling Transferrin Receptor with db14195. Counter stained with Hematoxylin. -
Immunohistochemical analysis of paraffin-embedded mouse renal tissue labeling Transferrin Receptor with db14195. Counter stained with Hematoxylin.
订购信息
- Package Price (RMB)
- 10μL 398
- 20μL 698
- 50μL 1398
- 100μL 2398
- 货期：现货
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For research use only. Not intended for diagnostic.
