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Conjugation/Formulation：Un-conjugated
- Background The protein encoded by this gene is a member of the MAP kinase family. MAP kinases act as an integration point for multiple biochemical signals, and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation and development. This kinase is activated by various cell stimuli, and targets specific transcription factors, and thus mediates immediate-early gene expression in response to cell stimuli. The activation of this kinase by tumor-necrosis factor alpha (TNF-alpha) is found to be required for TNF-alpha induced apoptosis. This kinase is also involved in UV radiation induced apoptosis, which is thought to be related to cytochrom c-mediated cell death pathway. Studies of the mouse counterpart of this gene suggested that this kinase play a key role in T cell proliferation, apoptosis and differentiation. Several alternatively spliced transcript variants encoding distinct isoforms have been reported. [provided by RefSeq, Apr 2016]
- Immunogen Recombinant protein of human JNK1
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Gene ID
5599
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Swiss Prot
P45983
- Synonyms JNK; JNK1; PRKM8; SAPK1; JNK-46; JNK1A2; SAPK1c; JNK21B1/2
- Reactivity Human,Mouse,Rat
- Application WB, ICC/IF, FC, IP
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Recommended dilution
WB: 1:1000
ICC/IF: 1:500-1:1000
FC: 1:100
IP: 1:20-1:50 - Calculated MW 48,53 kDa
- Observed MW 46,54 kDa
- Host species Rabbit
- Clonality Monoclonal
- Clonality No. DGR13729
- Isotype IgG
- Purity Affinity Purification
- Conjugation Un-conjugated
- Storage/Stability Store at -20°C. Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA. Stable for 12 months from date of receipt.
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Western blot analysis of extracts from C6 cells and Rat brain tissue using db14225 at 1:1000. -
Western blot analysis of extracts from Jurkat cells using db14225 at 1:1000. -
Western blot analysis of extracts from HeLa cells using db14225 at 1:1000. -
Western blot analysis of extracts from Mouse brain tissue using db14225 at 1:1000. -
Immunofluorescence analysis of HeLa cells labelling JNK1/JNK2/JNK3 with db14225.
The cells were fixed with cold 100% methanol (10min, 4℃) and blocked in 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween 20 for 1h. The cells were then incubate with db14225 (1:500) at room temprature for 1h, followed by a further incubation at room temperature for 45min with Goat Anti Rabbit lgG (H+L)-AF488 (db10005, shown in green). Nuclear DNA was labeled in blue with DAPI.
Control: Secondary antibody only.
订购信息
- Package Price (RMB)
- 10μL 398
- 20μL 698
- 50μL 1398
- 100μL 2398
- 货期：现货
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For research use only. Not intended for diagnostic.
