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Conjugation/Formulation：Un-conjugated
- Background The protein encoded by this gene is a member of the TNF-receptor superfamily, and contains an intracellular death domain. This receptor can be activated by tumor necrosis factor-related apoptosis inducing ligand (TNFSF10/TRAIL/APO-2L), and transduces an apoptosis signal. Studies with FADD-deficient mice suggested that FADD, a death domain containing adaptor protein, is required for the apoptosis mediated by this protein. Two transcript variants encoding different isoforms and one non-coding transcript have been found for this gene. [provided by RefSeq, Mar 2009]
- Immunogen Recombinant protein of human DR5
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Gene ID
8795
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Swiss Prot
O14763
- Synonyms DR5; CD262; KILLER; TRICK2; TRICKB; ZTNFR9; TRAILR2; TRICK2A; TRICK2B; TRAIL-R2; KILLER/DR5
- Reactivity Human
- Application WB, ICC/IF, FC, IP
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Recommended dilution
WB: 1:1000
ICC/IF: 1:100
FC: 1:50-1:100
IP: 1:20-1:50 - Calculated MW 49 kDa
- Observed MW 40,49 kDa
- Host species Rabbit
- Clonality Monoclonal
- Clonality No. DGR19548
- Isotype IgG
- Purity Affinity Purification
- Conjugation Un-conjugated
- Storage/Stability Store at -20°C. Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA. Stable for 12 months from date of receipt.
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Western blot analysis of extracts from LNCaP cells using db14494 at 1:1000. -
Western blot analysis of extracts from HepG2, HCT116, U2OS cells using db14494 at 1:1000. -
Immunofluorescence analysis of HeLa cells labelling DR5 with db14494.
The cells were fixed with 4% PFA (10min, RT) followed by treatment with 0.1% Triton X-100 (10min, RT), and blocked in 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween 20 for 1h. The cells were then incubate with db14494 (1:100) at room temprature for 1h, followed by a further incubation at room temperature for 45min with Goat Anti Rabbit lgG (H+L)-AF488 (db10005, shown in green). Nuclear DNA was labeled in blue with DAPI.
Control: Secondary antibody only.
订购信息
- Package Price (RMB)
- 10μL 398
- 20μL 698
- 50μL 1398
- 100μL 2398
- 货期：现货
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For research use only. Not intended for diagnostic.
