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Conjugation/Formulation：Un-conjugated
- Background The protein encoded by this gene is a member of the S100 family of proteins containing 2 EF-hand calcium-binding motifs. S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. S100 genes include at least 13 members which are located as a cluster on chromosome 1q21. This protein may function in motility, invasion, and tubulin polymerization. Chromosomal rearrangements and altered expression of this gene have been implicated in tumor metastasis. Multiple alternatively spliced variants, encoding the same protein, have been identified. [provided by RefSeq, Jul 2008]
- Immunogen A synthetic peptide of human S100A4
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Gene ID
6275
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Swiss Prot
P26447
- Synonyms 42A; 18A2; CAPL; FSP1; MTS1; P9KA; PEL98
- Reactivity Human
- Application WB, IHC-P, ICC/IF, FC, IP
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Recommended dilution
WB: 1:1000-1:5000
IHC-P: 1:200-1:500
ICC/IF: 1:100-1:200
FC: 1:100-1:500
IP: 1:10-1:100 - Calculated MW 12 kDa
- Observed MW 12 kDa
- Host species Rabbit
- Clonality Monoclonal
- Clonality No. DGR12310
- Isotype IgG
- Purity Affinity Purification
- Conjugation Un-conjugated
- Storage/Stability Store at -20°C. Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA. Stable for 12 months from date of receipt.
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Western blot analysis of extracts from HeLa cells using db14618 at 1:1000. -
Immunohistochemical analysis of paraffin-embedded human lung cancer using db14618 antibody. -
Immunofluorescent analysis of HeLa cells using db14618 antibody (green), and DAPI (blue). -
Immunofluorescence analysis of A549 cells labelling S100A4 with db14618.
The cells were fixed with cold 100% methanol (10min, 4℃) and blocked in 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween 20 for 1h. The cells were then incubate with db14618 (1:100) at room temprature for 1h, followed by a further incubation at room temperature for 45min with Goat Anti Rabbit lgG (H+L)-AF488 (db10005, shown in green). Nuclear DNA was labeled in blue with DAPI.
Control: Secondary antibody only.
订购信息
- Package Price (RMB)
- 10μL 398
- 20μL 698
- 50μL 1398
- 100μL 2398
- 货期：现货
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For research use only. Not intended for diagnostic.
