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GSK3 alpha (DGR15531) Rabbit mAb
db14872
Datasheet
- Background This gene encodes a multifunctional Ser/Thr protein kinase that is implicated in the control of several regulatory proteins including glycogen synthase, and transcription factors, such as JUN. It also plays a role in the WNT and PI3K signaling pathways, as well as regulates the production of beta-amyloid peptides associated with Alzheimer's disease. [provided by RefSeq, Oct 2011]
- Immunogen A synthetic peptide of human GSK3 alpha
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Gene ID
2931
-
Swiss Prot
P49840
- Synonyms Serine/threonine-protein kinase GSK3A; GSK3A
- Reactivity Human,Mouse,Rat
- Application WB, IHC-P, ICC/IF
-
Recommended dilution
WB: 1:1000-1:5000
IHC-P: 1:50
ICC/IF: 1:100-1:200 - Calculated MW 51 kDa
- Observed MW 51 kDa
- Host species Rabbit
- Clonality Monoclonal
- Clonality No. DGR15531
- Isotype IgG
- Purity Affinity Purification
- Conjugation Un-conjugated
- Storage/Stability Store at -20°C. Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA. Stable for 12 months from date of receipt.
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Western blot detection of GSK3 alpha in K562,Rat Brain,Hela cell lysates using GSK3 alpha antibody(1:1000 diluted). -
Immunofluorescent analysis of 293T cells using db14872 antibody (green), and DAPI (blue). -
Western blot analysis of GSK3 alpha in mouse brain lysates using GSK3 alpha antibody(1:1000 diluted). -
Immunofluorescence analysis of HeLa cells labelling GSK3 alpha with db14872.
The cells were fixed with 4% PFA (10min, RT) followed by treatment with 0.1% Triton X-100 (10min, RT), and blocked in 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween 20 for 1h. The cells were then incubate with db14872 (1:100) at room temprature for 1h, followed by a further incubation at room temperature for 45min with Goat Anti Rabbit lgG (H+L)-AF488 (db10005, shown in green). Nuclear DNA was labeled in blue with DAPI.
Control: Secondary antibody only.
订购信息
- Package Price (RMB)
- 10μL 398
- 20μL 698
- 50μL 1398
- 100μL 2398
- 货期：现货
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For research use only. Not intended for diagnostic.
