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SIRT1 (DGR13955) Rabbit mAb
db15473
Datasheet
- Background This gene encodes a member of the sirtuin family of proteins, homologs to the yeast Sir2 protein. Members of the sirtuin family are characterized by a sirtuin core domain and grouped into four classes. The functions of human sirtuins have not yet been determined; however, yeast sirtuin proteins are known to regulate epigenetic gene silencing and suppress recombination of rDNA. Studies suggest that the human sirtuins may function as intracellular regulatory proteins with mono-ADP-ribosyltransferase activity. The protein encoded by this gene is included in class I of the sirtuin family. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2008]
- Immunogen A synthetic peptide of human SIRT1
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Gene ID
23411
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Swiss Prot
Q96EB6
- Synonyms SIR2; SIR2L1; SIR2alpha
- Reactivity Human
- Application WB, IHC-P, ICC/IF, FC, IP
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Recommended dilution
WB: 1:2000-1:20000
IHC-P: 1:100-1:200
ICC/IF: 1:100
FC: 1:100-1:200
IP: 1:20-1:50 - Calculated MW 82 kDa
- Observed MW 120 kDa
- Host species Rabbit
- Clonality Monoclonal
- Clonality No. DGR13955
- Isotype IgG
- Purity Affinity Purification
- Conjugation Un-conjugated
- Storage/Stability Store at -20°C. Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA. Stable for 12 months from date of receipt.
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Western blot analysis of extracts from HeLa cells using db15473 at 1:1000. -
Immunofluorescence analysis of HeLa cells labelling SIRT1 with db15473.
The cells were fixed with cold 100% methanol (10min, 4℃) and blocked in 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween 20 for 1h. The cells were then incubate with db15473 (1:200) at room temprature for 1h, followed by a further incubation at room temperature for 45min with Goat Anti Rabbit lgG (H+L)-AF488 (db10005, shown in green). Nuclear DNA was labeled in blue with DAPI.
Control: Secondary antibody only.
订购信息
- Package Price (RMB)
- 10μL 398
- 20μL 698
- 50μL 1398
- 100μL 2398
- 货期：现货
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For research use only. Not intended for diagnostic.
