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IRS1 (DGR13608) Rabbit mAb
db15530
Datasheet
- Background This gene encodes a protein which is phosphorylated by insulin receptor tyrosine kinase. Mutations in this gene are associated with type II diabetes and susceptibility to insulin resistance. [provided by RefSeq, Nov 2009]
- Immunogen A synthetic peptide of human IRS1
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Gene ID
3667
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Swiss Prot
P35568
- Synonyms HIRS-1
- Reactivity Human
- Application WB, IHC, ICC/IF, FC
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Recommended dilution
WB: 1:1000-1:5000
IHC: 1:20-1:50
ICC/IF: 1:50-1:200
FC: 1:20 - Calculated MW 132 kDa
- Observed MW 180 kDa
- Host species Rabbit
- Clonality Monoclonal
- Clonality No. DGR13608
- Isotype IgG
- Purity Affinity Purification
- Conjugation Un-conjugated
- Storage/Stability Store at -20°C. Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA. Stable for 12 months from date of receipt.
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Immunofluorescence analysis of HeLa cells labelling IRS1 with db15530.
The cells were fixed with cold 100% methanol (10min, 4℃) and blocked in 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween 20 for 1h. The cells were then incubate with db15530 (1:50) at room temprature for 1h, followed by a further incubation at room temperature for 45min with Goat Anti Rabbit lgG (H+L)-AF488 (db10005, shown in green). Nuclear DNA was labeled in blue with DAPI.
Control: Secondary antibody only.
订购信息
- Package Price (RMB)
- 10μL 398
- 20μL 698
- 50μL 1398
- 100μL 2398
- 货期：现货
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For research use only. Not intended for diagnostic.
