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KDM5A/Jarid1A/RBBP2 (DGR11614) Rabbit mAb
db15949
Datasheet
- Background This gene encodes a member of the Jumonji, AT-rich interactive domain 1 (JARID1) histone demethylase protein family. The encoded protein plays a role in gene regulation through the histone code by specifically demethylating lysine 4 of histone H3. The encoded protein interacts with many other proteins, including retinoblastoma protein, and is implicated in the transcriptional regulation of Hox genes and cytokines. This gene may play a role in tumor progression. [provided by RefSeq, Aug 2013]
- Immunogen Recombinant protein of human KDM5A/Jarid1A/RBBP2
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Gene ID
5927
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Swiss Prot
P29375
- Synonyms RBP2; RBBP2; RBBP-2
- Reactivity Human,Mouse
- Application WB, ICC/IF, FC, IP
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Recommended dilution
WB: 1:1000-1:5000
ICC/IF: 1:500-1:2000
FC: 1:200-1:1000
IP: 1:100 - Calculated MW 192 kDa
- Observed MW 192 kDa
- Host species Rabbit
- Clonality Monoclonal
- Clonality No. DGR11614
- Isotype IgG
- Purity Affinity Purification
- Conjugation Un-conjugated
- Storage/Stability Store at -20°C. Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA. Stable for 12 months from date of receipt.
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Western blot analysis of extracts from A431 cells using db15949 at 1:1000. -
Immunofluorescence analysis of HeLa cells labelling KDM5A/Jarid1A/RBBP2 with db15949.
The cells were fixed with 4% PFA (10min, RT) followed by treatment with 0.1% Triton X-100 (10min, RT), and blocked in 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween 20 for 1h. The cells were then incubate with db15949(1:2000) at room temprature for 1h, followed by a further incubation at room temperature for 45min with Goat Anti Rabbit lgG (H+L)-AF488 (db10005, shown in green). Nuclear DNA was labeled in blue with DAPI.
Control: Secondary antibody only.
订购信息
- Package Price (RMB)
- 10μL 398
- 20μL 698
- 50μL 1398
- 100μL 2398
- 货期：现货
相关产品
For research use only. Not intended for diagnostic.
