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PI3-Kinase p85 alpha (DGR16380) Rabbit mAb
db16082
Datasheet
- Background Phosphatidylinositol 3-kinase phosphorylates the inositol ring of phosphatidylinositol at the 3-prime position. The enzyme comprises a 110 kD catalytic subunit and a regulatory subunit of either 85, 55, or 50 kD. This gene encodes the 85 kD regulatory subunit. Phosphatidylinositol 3-kinase plays an important role in the metabolic actions of insulin, and a mutation in this gene has been associated with insulin resistance. Alternative splicing of this gene results in four transcript variants encoding different isoforms. [provided by RefSeq, Jun 2011]
- Immunogen A synthetic peptide of human PI 3 Kinase p85 alpha
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Gene ID
5295
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Swiss Prot
P27986
- Synonyms p85; AGM7; GRB1; IMD36; p85-ALPHA
- Reactivity Human, Mouse, Rat
- Application IHC, ICC/IF, FC
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Recommended dilution
IHC: 1:100
ICC/IF: 1:50
FC: 1:50 - Calculated MW 84 kDa
- Observed MW 85 kDa
- Host species Rabbit
- Clonality Monoclonal
- Clonality No. DGR16380
- Isotype IgG
- Purity Affinity Purification
- Conjugation Un-conjugated
- Storage/Stability Store at -20°C. Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA. Stable for 12 months from date of receipt.
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Immunofluorescence analysis of 3T3 cells labelling PI3-Kinase p85 alpha with db16082.
The cells were fixed with cold 100% methanol (10min, 4℃) and blocked in 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween 20 for 1h. The cells were then incubate with db16082 (1:50) at room temprature for 1h, followed by a further incubation at room temperature for 45min with Goat Anti Rabbit lgG (H+L)-AF488 (db10005, shown in green). Nuclear DNA was labeled in blue with DAPI.
Control: Secondary antibody only.
订购信息
- Package Price (RMB)
- 10μL 398
- 20μL 698
- 50μL 1398
- 100μL 2398
- 货期：现货
相关产品
For research use only. Not intended for diagnostic.
