SLC1A5单克隆抗体(DGR19941) | 高特异性&高亲和力

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Recombinant DGRmAb^®

SLC1A5 (DGR19941) Rabbit mAb

db16157

Datasheet

Product Name : SLC1A5 (DGR19941) Rabbit mAb

Cat.No.: db16157

Synonyms : R16; AAAT; ATBO; M7V1; RDRC; ASCT2; M7VS1

Application : WB, IHC-P, ICC/IF, FC, IP

Reactivity : Human

Host species : Rabbit

Background The SLC1A5 gene encodes a sodium-dependent neutral amino acid transporter that can act as a receptor for RD114/type D retrovirus (Larriba et al., 2001 [PubMed 11781704]).[supplied by OMIM, Jan 2011]
Immunogen A synthetic peptide of human SLC1A5
Gene ID 6510
Swiss Prot Q15758
Synonyms R16; AAAT; ATBO; M7V1; RDRC; ASCT2; M7VS1
Reactivity Human
Application WB, IHC-P, ICC/IF, FC, IP
Recommended dilution WB: 1:1000
IHC-P: 1:200-1:2000
ICC/IF: 1:50
FC: 1:20-1:50
IP: 1:20-1:50
Calculated MW 57 kDa
Observed MW 70-75 kDa
Host species Rabbit
Clonality Monoclonal
Clonality No. DGR19941
Isotype IgG
Purity Affinity Purification
Conjugation Un-conjugated
Storage/Stability Store at -20°C. Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA. Stable for 12 months from date of receipt.

Immunofluorescence analysis of Hela cells labelling SLC1A5 with db16157.

The cells were fixed with cold 100% methanol (10min, 4℃) and blocked in 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween 20 for 1h. The cells were then incubate with db16157 (1:50) at room temprature for 1h, followed by a further incubation at room temperature for 45min with Goat Anti Rabbit lgG (H+L)-AF488 (db10005, shown in green). Nuclear DNA was labeled in blue with DAPI.

Control: Secondary antibody only.
Immunofluorescence analysis of HeLa cells labelling SLC1A5 with db16157.

The cells were fixed with 4% PFA (10min, RT) followed by treatment with 0.1% Triton X-100 (10min, RT), and blocked in 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween 20 for 1h. The cells were then incubate with db16157 (1:50) at room temprature for 1h, followed by a further incubation at room temperature for 45min with Goat Anti Rabbit lgG (H+L)-AF647(db10006, shown in red). Nuclear DNA was labeled in blue with DAPI.

Control: Secondary antibody only.

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